| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05787-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09027-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62564-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50231-0.025ml | |||
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0003.jpg "Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human pancreas with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0001.jpg)
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human ovarian cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0002.jpg)
![Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: A549 treated with pp for 1 hour cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 110/140 kDa<br/><br/> Exposure time: 57 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_rabbit-mdm2-hdm2-p-ser166-mab-je64-20-288202412461655.jpg "Western Blot: MDM2/HDM2 [p Ser166] Antibody (JE64-20) [NBP3-32777] - Western blot analysis of MDM2/HDM2 on different lysates with Rabbit anti-MDM2/HDM2 antibody (NBP3-32777) at 1/1,000 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: A549 treated with pp for 1 hour cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 55 kDa<br/> Observed band size: 110/140 kDa<br/><br/> Exposure time: 57 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-32777 at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with 1μg/mL insulin for 2.5 hours cell lysate Lane 3: A549 treated with 1μg/mL insulin for 2.5 hours cell lysate, then the membrane treated with lambda pp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 110/140 kDa Exposure time: 57 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32777 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32777_-western-blot-639204597138504533.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg "Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Western blot analysis on different lysates with NBP3-32782 at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5μM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5μM Doxorubicin for 24 hours, then treated with lambda pp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32782 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_-western-blot-639204595248046518.jpg)
![Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_-western-blot-639204590075999270.jpg "Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-32317 at 1/1,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32317 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG [HRP] Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_-western-blot-639204597793077982.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Immunochemical staining of human IRE1 alpha in human epididymis with NBP3-05787 (1:100, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-05787_mouse-monoclonal-ire1-alpha-antibody-10b2b2-23520251628119.png "Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Immunochemical staining of human IRE1 alpha in human epididymis with NBP3-05787 (1:100, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Immunochemical staining of human IRE1 alpha in human tonsil with NBP3-05787 (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-05787_mouse-monoclonal-ire1-alpha-antibody-10b2b2-23520251544312.png)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Immunochemical staining of human IRE1 alpha in human liver with NBP3-05787 (1:300, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-05787_mouse-monoclonal-ire1-alpha-antibody-10b2b2-235202516144319.png)
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0005.jpg "Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).")
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0004.jpg)
![Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/IL-16-Antibody-14-1-Chimeric-Flow-Cytometry-NBP3-09027-img0001.jpg "Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.")
![Western Blot: iNOS Antibody [NBP3-23216] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23216_rabbit-inos-pab-199202312523648.jpg "<b>Biological Strategies Validation. </b>Western Blot: iNOS Antibody [NBP3-23216] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).")
![Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.](http://images.novusbio.com/fullsize/p53-Antibody-V5P4H6-B3-Immunohistochemistry-NBP2-50231-img0001.jpg "Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.")