| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26215-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26400-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26425-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26154-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26186-50ul | |||
![Western Blot: iNOS Antibody (M398) [NBP3-23204] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23204_mouse-inos-mab-m398-19920231410117.jpg "<b>Biological Strategies Validation. </b>Western Blot: iNOS Antibody (M398) [NBP3-23204] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).")
![Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-262202414471543.jpg "Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-26220241422529.jpg)
![Flow Cytometry: HSP90 beta Antibody (7F4) [NBP3-26215] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7F4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-282202414183699.jpg)
![Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-282202411363013.jpg "<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Staining of A549 cells with AMPK alpha 2 [p Thr172] Antibody (4F4) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-28220241323610.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-28220241147219.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Staining of Hela cells with IRE1 alpha [p Ser724] Antibody (1F12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-282202412475125.jpg)
![Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-28220241144268.jpg "Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Flow Cytometry: LC3B Antibody (7D11) [NBP3-26154] - Overlay Peak curve showing Hela cells stained with LC3B Antibody (7D11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-282202414183621.jpg)
![Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-262202414471587.jpg "Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: c-jun Antibody (10D4) [NBP3-26186] - Positive Western Blot detected in: 293 whole cell lysate. All lanes: c-jun Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa Observed band size: 43 kDa](http://images.novusbio.com/fullsize/nbp3-26186_rabbit-c-jun-mab-10d4-28220241144263.jpg)