| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49516-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05599-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11591 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11592 | |||
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human testis shows moderate membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0008.jpg)
![Immunohistochemistry: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human placenta shows membranous positivity in trophoblasts.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-NBP2-49516-img0001.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0006.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0007.jpg)
![ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase IX/CA9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-71283_mouse-carbonic-anhydrase-ix-ca9-elisa-kit-colorimetric-132202416232325.jpg "ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase IX/CA9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-71283_mouse-carbonic-anhydrase-ix-ca9-elisa-kit-colorimetric-432024973616.jpg)
![Western Blot: COX-1 Antibody [NBP3-05599] - Western blot analysis of extracts of mouse skeletal muscle, using COX-1 antibody (NBP3-05599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP3-05599-img0002.jpg "Western Blot: COX-1 Antibody [NBP3-05599] - Western blot analysis of extracts of mouse skeletal muscle, using COX-1 antibody (NBP3-05599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: COX-1 Antibody - Azide and BSA Free [COX-1] - Immunohistochemistry analysis of paraffin-embedded Human liver using COX-1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251640246.jpg)
![Western Blot: COX-1 Antibody - Azide and BSA Free [COX-1] - Western blot analysis of lysates from NIH/3T3 cells using COX-1 Rabbit pAb at 1:1800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251640224.jpg)
![Western Blot: COX-1 Antibody - Azide and BSA Free [COX-1] - Western blot analysis of lysates from NIH/3T3 cells, using COX-1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251638087.jpg)
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western blot analysis on different lysates with NBP3-32017 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: U-2 OS cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32017 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_-western-blot-639204590070210928.jpg)
![Western blot analysis on different lysates with NBP3-32017 at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: A549 cell lysate Lane 3: U-2 OS cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: PC-12 cell lysate Lane 9: Mouse brain tissue lysate Lane 10: Mouse heart tissue lysate Lane 11: Mouse testis tissue lysate Lane 12: Rat brain tissue lysate Lane 13: Rat heart tissue lysate Lane 14: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32017 at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_-western-blot-639204597791956948.jpg)
![Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NB100-87001-img0004.jpg "Western Blot: CREB Antibody [NB100-87001] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-87001 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Immunohistochemistry-Paraffin: CREB Antibody [NB100-87001] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CREB used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-NB100-87001-img0013.jpg)
![Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Western-Blot-NBP1-77944-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Immunohistochemistry-NBP1-77944-img0006.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Western-Blot-NBP1-77936-img0003.jpg "Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.")
![Immunohistochemistry-Paraffin: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Tissue: placental trophoblasts. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: EGFR pY1197 antibody at 5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: EGFR pY1197 is on the cell membrane. Staining: EGFR pY1197 as precipitated red signal with hematoxylin purple counterstain.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Immunohistochemistry-Paraffin-NBP1-77936-img0002.jpg)
![Western Blot: AKT2 Antibody (11F6.B2) [NBP2-44216] - lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 Ug per lane.](http://images.novusbio.com/fullsize/AKT2-Antibody-11F6-B2-Western-Blot-NBP2-44216-img0003.jpg "Western Blot: AKT2 Antibody (11F6.B2) [NBP2-44216] - lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 Ug per lane.")
![Western Blot: AKT2 Antibody (11F6.B2) [NBP2-44216] - lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/AKT2-Antibody-11F6-B2-Western-Blot-NBP2-44216-img0002.jpg)
![ELISA: AKT2 Antibody (11F6.B2) [NBP2-44216] - ELISA of Rat Monoclonal anti-AKT2 Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well.](http://images.novusbio.com/fullsize/AKT2-Antibody-11F6-B2-ELISA-NBP2-44216-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Immunohistochemistry-Paraffin-NBP2-45237-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).")
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781). As indicated by the arrow, a specific band was detected at a molecular weight of approximately 50 kDa.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Western-Blot-NBP2-45237-img0002.jpg)
![Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP1-19102-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-19102-img0003.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-PN-15-Immunohistochemistry-Paraffin-NBP2-45238-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).")
![Western Blot: Myosin Heavy Chain 1 Antibody - BSA Free [NBP3-46984] - Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with (Myosin Heavy Chain 1 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-46984_rabbit-myosin-heavy-chain-1-pab-bsa-free-284202512315788.jpg "Western Blot: Myosin Heavy Chain 1 Antibody - BSA Free [NBP3-46984] - Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with (Myosin Heavy Chain 1 Antibody) at dilution of 1:1000")
![Immunoprecipitation: Myosin Heavy Chain 1 Antibody - BSA Free [NBP3-46984] - IP Result of anti-Myosin Heavy Chain 1 (IP:, 5ug; Detection: 1:1000) with mouse skeletal muscle tissue lysate 4000ug.](http://images.novusbio.com/fullsize/nbp3-46984_rabbit-myosin-heavy-chain-1-pab-bsa-free-284202512313656.jpg)
![Immunohistochemistry: Myosin Heavy Chain 1 Antibody - BSA Free [NBP3-46984] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (Myosin Heavy Chain 1 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-46984_rabbit-myosin-heavy-chain-1-pab-bsa-free-284202512313649.jpg)