| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92792-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95089-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37423-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52513-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89890-50ul | |||
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP2-92792] - Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (NBP2-92792) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP2-92792-img0003.jpg "Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP2-92792] - Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (NBP2-92792) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: BNIP3L Antibody [NBP2-92792] - Immunohistochemistry of paraffin-embedded mouse lung using BNIP3L Rabbit pAb (NBP2-92792) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BNIP3L-Antibody-Immunohistochemistry-Paraffin-NBP2-92792-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: BNIP3L Antibody - Azide and BSA Free [NBP2-92792] - Immunofluorescence analysis of NIH/3T3 cells using BNIP3L Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92792_rabbit-polyclonal-bnip3l-antibody-42202514275234.jpg)
![Western Blot: BNIP3L Antibody - Azide and BSA Free [NBP2-92792] - Western blot analysis of lysates from PC-12 cells using BNIP3L Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-92792_rabbit-polyclonal-bnip3l-antibody-42202514264491.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Western-Blot-NBP2-95089-img0005.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human prostate using ATP1A1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95089-img0001.jpg)
![Immunoprecipitation: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of 200ug extracts of LO2 cells, using Sodium Potassium ATPase Alpha 1 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunoprecipitation-NBP2-95089-img0003.jpg)
![Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Western-Blot-NBP3-15366-img0004.jpg "Western Blot: IDH2 Antibody (8C3D4) [NBP3-15366] - Western blot analysis of extracts of various cell lines, using IDH2 Rabbit mAb (NBP3-15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of NIH-3T3 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0003.jpg)
![Immunohistochemistry-Paraffin: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunohistochemistry-Paraffin-NBP3-15366-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IDH2 Antibody (8C3D4) [NBP3-15366] - Immunofluorescence analysis of C6 cells using IDH2 Rabbit mAb (NBP3-15366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IDH2-Antibody-8C3D4-Immunocytochemistry-Immunofluorescence-NBP3-15366-img0002.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Western-Blot-NBP2-37423-img0003.jpg "Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis of endometrial cancer tissues using SPP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Immunohistochemistry-NBP2-37423-img0005.jpg)
![Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Western-Blot-NBP2-37423-img0002.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis of prostate cancer tissues using SPP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-Immunohistochemistry-NBP2-37423-img0004.jpg)
![ELISA: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-7C5H12-ELISA-NBP2-37423-img0001.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0003.jpg "Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)")
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of endometrial cancer tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0002.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against HEK293 (1) and mTOR (AA: 2311-2529)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0004.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of brain tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0001.jpg)
![ELISA: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-ELISA-NBP2-52513-img0005.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29820241420955.jpg "Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33143, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33143) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-298202413585572.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Immunocytochemistry analysis of HeLa cells labeling Sodium Potassium ATPase Alpha 1 with Rabbit anti-Sodium Potassium ATPase Alpha 1 Alpha 1 antibody (NBP3-33143) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-29202410234287.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (ST0533) [NBP3-33143] - Western blot analysis of Sodium Potassium ATPase Alpha 1 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 1 antibody (NBP3-33143) at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33143_rabbit-sodium-potassium-atpase-alpha-1-mab-st0533-392024848504.jpg)
![Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Western-Blot-NBP2-89890-img0003.jpg "Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunohistochemistry-Paraffin-NBP2-89890-img0001.jpg)
![Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate 2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunoprecipitation-NBP2-89890-img0004.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human ORP150/HSP12A in human colon carcinoma with NBP2-89890 (1:200).](http://images.novusbio.com/fullsize/nbp2-89890_rabbit-monoclonal-orp150-hsp12a-antibody-201-235202514492364.png)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89890 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89890_rabbit-monoclonal-orp150-hsp12a-antibody-201-23520251664729.png)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: AKT2 Antibody (95C657.1.2) [NB100-56676] - analysis of Akt2 using this antibody.](http://images.novusbio.com/fullsize/AKT2-Antibody-95C657-1-2-Western-Blot-NB100-56676-img0002.jpg "Western Blot: AKT2 Antibody (95C657.1.2) [NB100-56676] - analysis of Akt2 using this antibody.")
![Flow Cytometry: AKT2 Antibody (95C657.1.2) [NB100-56676] - An intracellular stain was performed on A549 cells with AKT2 Antibody (95C657.1.2) NB100-56676 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgM (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/AKT2-Antibody-95C657-1-2-Flow-Cytometry-NB100-56676-img0003.jpg)