| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94151-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16955-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29664-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1312-0.025ml | |||
![Western Blot: TRIB3 Antibody - BSA Free [TRIB3] - Western blot analysis of lysates from Mouse liver using TRIB3 Rabbit pAb at 1:1000 dilution. AS014Secondary antibody: at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:(Mouse).Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp2-94151_rabbit-polyclonal-trib3-antibody-5220251636327.jpg "Western Blot: TRIB3 Antibody - BSA Free [TRIB3] - Western blot analysis of lysates from Mouse liver using TRIB3 Rabbit pAb at 1:1000 dilution. AS014Secondary antibody: at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:(Mouse).Exposure time: 120s.")
![Western Blot: TOR/mTOR [p Ser2481] Antibody (9Y5P6) [NBP3-16907] - Western blot analysis of extracts of various cell lines, using TOR/mTOR (NBP3-16907) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TOR-mTOR-p-Ser2481-Antibody-9Y5P6-Western-Blot-NBP3-16907-img0001.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (9Y5P6) [NBP3-16907] - Western blot analysis of extracts of various cell lines, using TOR/mTOR (NBP3-16907) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-0G10F8-Western-Blot-NBP3-16404-img0001.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (0G10F8) [NBP3-16404] - Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase I/CA1 (CA1) Rabbit mAb (NBP3-16404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p53-[p-Thr81]-Antibody-Western-Blot-NBP3-16955-img0001.jpg "Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/p53-[p-Ser392]-Antibody-Western-Blot-NB200-156-img0001.jpg "Western Blot: p53 [p Ser392] Antibody [NB200-156] - rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k p53 phosphorylated Rat Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/COX-IV-[p-Ser58]-Antibody-Western-Blot-NBP2-29532-img0001.jpg "Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).](http://images.novusbio.com/fullsize/eNOS-[p-Ser116]-Antibody-Western-Blot-NBP2-29664-img0002.jpg "Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).")
![Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745] - Western blot analysis of EGLN3/PHD3 on different lysates with Rabbit anti-EGLN3/PHD3 antibody (NBP3-32745) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate (40 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: A375 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32745_rabbit-egln3-phd3-mab-je60-82-27820241631688.jpg "Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745] - Western blot analysis of EGLN3/PHD3 on different lysates with Rabbit anti-EGLN3/PHD3 antibody (NBP3-32745) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate (40 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: A375 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32812_rabbit-tsc2-p-ser939-mab-je54-06-28820241244294.jpg "Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IRP2 Antibody [NBP3-10538] - Host: Rabbit. Target Name: IRP2. Sample Tissue: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/IRP2 Antibody-Western Blot-NBP3-10538-img0001.jpg "Western Blot: IRP2 Antibody [NBP3-10538] - Host: Rabbit. Target Name: IRP2. Sample Tissue: Human Fetal Lung. Antibody Dilution: 1.0ug/ml")
![Western Blot: iNOS Antibody [NBP2-42880] - Untreated (--) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP2-42880-img0004.jpg "Western Blot: iNOS Antibody [NBP2-42880] - Untreated (--) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")