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![Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP1-36985-img0006.jpg "Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-36985-img0007.jpg)
![Western Blot: ICA1 Antibody [NBP1-56426] - Human HepG2, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that ICA1 is expressed in HepG2.](http://images.novusbio.com/fullsize/ICA1-Antibody-Western-Blot-NBP1-56426-img0004.jpg "Western Blot: ICA1 Antibody [NBP1-56426] - Human HepG2, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that ICA1 is expressed in HepG2.")
![Immunohistochemistry: ICA1 Antibody [NBP1-56426] - Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Tim](http://images.novusbio.com/fullsize/ICA1-Antibody-Immunohistochemistry-NBP1-56426-img0003.jpg)
![Western Blot: ICA1 Antibody [NBP1-56426] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ICA1-Antibody-Western-Blot-NBP1-56426-img0002.jpg)
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0003.jpg "Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: FKBP38 Antibody (2J7P6) [NBP3-16684] - Western blot analysis of extracts of various cell lines, using FKBP38 Rabbit mAb (NBP3-16684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FKBP38-Antibody-2J7P6-Western-Blot-NBP3-16684-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FKBP38 Antibody (2J7P6) [NBP3-16684] - Confocal imaging of NIH/3T3 cells using FKBP38 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16684_rabbit-fkbp38-mab-2j7p6-8220252010492.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0003.jpg "Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Immunocytochemistry-Immunofluorescence-NBP2-50579-img0004.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0001.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0002.jpg)
![Western Blot: PSENEN/PEN2 Antibody (6J5O4) [NBP3-16676] - Western blot analysis of extracts of various cell lines, using PSENEN/PEN2 Rabbit mAb (NBP3-16676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-6J5O4-Western-Blot-NBP3-16676-img0002.jpg "Western Blot: PSENEN/PEN2 Antibody (6J5O4) [NBP3-16676] - Western blot analysis of extracts of various cell lines, using PSENEN/PEN2 Rabbit mAb (NBP3-16676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: PSENEN/PEN2 Antibody (6J5O4) [NBP3-16676] - Confocal imaging of NIH/3T3 cells using PSENEN/PEN2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16676_rabbit-psenen-pen2-mab-6j5o4-5220251255514.jpg)
![Immunocytochemistry/ Immunofluorescence: PSENEN/PEN2 Antibody (6J5O4) [NBP3-16676] - Confocal imaging of paraffin-embedded Mouse brain using PSENEN/PEN2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16676_rabbit-psenen-pen2-mab-6j5o4-52202511365169.jpg)
![Western Blot: Adenosine A1R Antibody [NBP3-15570] - Western blot analysis of extracts of Mouse heart, using Adenosine A1R antibody (NBP3-15570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Western-Blot-NBP3-15570-img0003.jpg "Western Blot: Adenosine A1R Antibody [NBP3-15570] - Western blot analysis of extracts of Mouse heart, using Adenosine A1R antibody (NBP3-15570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NBP3-15570] - Immunohistochemistry of paraffin-embedded human esophageal using Adenosine A1R Rabbit pAb (NBP3-15570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Immunohistochemistry-Paraffin-NBP3-15570-img0002.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NBP3-15570] - Immunohistochemistry of paraffin-embedded rat brain using Adenosine A1R Rabbit pAb (NBP3-15570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Immunohistochemistry-Paraffin-NBP3-15570-img0001.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NB100-665-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]")
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0003.jpg "Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.")
![Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Flow-Cytometry-NBP2-37451-img0004.jpg)
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0002.jpg)
![ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-ELISA-NBP2-37451-img0001.jpg)
![Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-288202412461662.jpg "Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg)
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/Smoothened-Antibody-Western-Blot-NBP3-06699-img0003.jpg "Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.")
![Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699] - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Smoothened-Antibody-Immunohistochemistry-Paraffin-NBP3-06699-img0008.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Western-Blot-NBP2-89210-img0001.jpg "Western Blot: Vimentin Antibody (001) [NBP2-89210] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:55 kDa")
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0002.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (001) [NBP2-89210] - Staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89210-img0003.jpg)