| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21627-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-20694 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74583 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35597-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12876-25ug | |||
![Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Western Blot of Bovine Serum Albumin antibody (5F9). Lane 1: Bovine Serum Albumin. Load: 50 ng per lane.Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.Block: 0.75% Casein for 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-Western-Blot-NB600-868-img0007.jpg "Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Western Blot of Bovine Serum Albumin antibody (5F9). Lane 1: Bovine Serum Albumin. Load: 50 ng per lane.Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.Block: 0.75% Casein for 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.")
![Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Bovine Serum Albumin Antibody (5F9) is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2).](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-Western-Blot-NB600-868-img0002.jpg)
![ELISA: Bovine Serum Albumin Antibody (5F9) [NB600-868] - ELISA Results of Bovine Serum Albumin antibody (5F9). Each well was coated in duplicate with 10 ug of Bovine Serum Albumin. The working dilution is 1:85,300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Rabbit Anti-Mouse HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-ELISA-NB600-868-img0008.jpg)
![Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21627_rabbit-gm130-golga2-mab-sr1125-5720231754852.jpg "Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.")
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody (SR1125) - BSA Free [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.](http://images.novusbio.com/fullsize/nbp3-21627_rabbit-gm130-golga2-mab-sr1125-103202523464518.jpg)
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249355572.jpg "Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects secreted ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249371598.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249432831.jpg)
![Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249351324.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0003.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Flow-Cytometry-NBP3-07740-img0001.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0002.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) [NBP2-02264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF .](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Western-Blot-NBP2-02264-img0006.jpg "Western Blot: Transferrin Antibody (OTI5G2) [NBP2-02264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF .")
![Immunocytochemistry/Immunofluorescence: Transferrin Antibody (OTI5G2) [NBP2-02264] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Transferrin.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Immunocytochemistry-Immunofluorescence-NBP2-02264-img0004.jpg)
![Flow Cytometry: Transferrin Antibody (OTI5G2) [NBP2-02264] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Transferrin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Flow-Cytometry-NBP2-02264-img0003.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) [NBP2-02264] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Western-Blot-NBP2-02264-img0005.jpg)
![Western Blot: Transferrin Antibody (OTI5G2) [NBP2-02264] - Analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Transferrin-Antibody-OTI5G2-Immunoprecipitation-NBP2-02264-img0007.jpg)
![Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS45-Antibody-Western-Blot-NB100-41372-img0002.jpg "Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0006.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Placenta.Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0005.jpg)
![Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Western-Blot-NBP2-82069-img0001.jpg "Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-82069] - Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82069-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 ug/ml.Red: ABCA7 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120241958468.gif)
![Immunohistochemistry: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-2711202419592055.gif)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120242022969.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody [NBP3-35597] - Immunofluorescence analysis of C6 cells using GOLGA5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35597_rabbit-golga5-pab-912202414213756.jpg "Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody [NBP3-35597] - Immunofluorescence analysis of C6 cells using GOLGA5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: GOLGA5 Antibody [NBP3-35597] - Western blot analysis of various lysates using GOLGA5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35597_rabbit-golga5-pab-912202414123873.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLGA5 Antibody [NBP3-35597] - Immunofluorescence analysis of L929 cells using GOLGA5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35597_rabbit-golga5-pab-912202415161874.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Western-Blot-NBP1-04346-img0007.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP1-04346-img0006.jpg)
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). Thesecondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-2H3-Flow-Cytometry-NBP1-04346-img0008.jpg)
![Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Western-Blot-NBP2-12876-img0007.jpg "Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Hsp40 HDJ1 visulaized using, tested on a human keratinocyte line HaCat. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Immunohistochemistry-Paraffin-NBP2-12876-img0004.jpg)
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg "Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)
![Western blot analysis on different lysates with NBP3-32446 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Mouse heart tissue lysate (40 µg/Lane) Lane 11: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32446 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_-western-blot-639204590075224668.jpg)