Bovine Serum Albumin Antibody (5F9) - BSA Free

Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Western Blot of Bovine Serum Albumin antibody (5F9). Lane 1: Bovine Serum Albumin. Load: 50 ng per lane.Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.Block: 0.75% Casein for 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.

Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Bovine Serum Albumin Antibody (5F9) is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2).

ELISA: Bovine Serum Albumin Antibody (5F9) [NB600-868] - ELISA Results of Bovine Serum Albumin antibody (5F9). Each well was coated in duplicate with 10 ug of Bovine Serum Albumin. The working dilution is 1:85,300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Rabbit Anti-Mouse HRP conjugated and TMB substrate.

Western Blot of Mouse anti-Bovine Serum Albumin Monoclonal Antibody. Lane 1: Bovine Serum Albumin. Load: 50 ng per lane. Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 0.75% Casein for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.

• Reactivity: Bv
• Applications: WB, DB, ELISA, IHC, IP
• Clone: 5F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Bovine Serum Albumin Antibody (5F9) - BSA Free Summary

• Description: Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Bovine Serum Albumin Antibody (5F9) was produced by repeated immunizations of balb/c mice with bovine serum albumin. (Uniprot: P02769)
• Localization: Secreted
• Specificity: The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:120000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested by ELISA, SDS-Page, and Western Blot. The antibody may be suitable for other applications, although no supporting data is available.
• Control:

  Human Serum Albumin Overexpression Lysate

Reactivity Notes

No cross reactivity is detected against other serum proteins by immunoblot

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Bovine Serum Albumin Antibody (5F9) - BSA Free

• albumin
• BSA
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB
• Entrez:
  • 280717(Bovine)
• Uniprot:
  • P02769(Bovine)