| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21451-0.025mg | |||
| BSA Free | NBP3-21451-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81085-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34782-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48274 | |||
| Azide and BSA Free | NBP3-11480 | |||
![Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-288202412442967.jpg "Western Blot: ROBO1 Antibody (10E2-R) [NBP3-32913] - Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (NBP3-32913) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 ug/Lane) Lane 2: NIH/3T3 cell lysate (10 ug/Lane) Lane 3: Rat brain tissue lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: RAW264.7 cell lysate (10 ug/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32913) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROBO1 Antibody (10E2-R) [NBP3-32913] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ROBO1 antibody (NBP3-32913) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32913) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32913_mouse-robo1-mab-10e2-r-29820241416189.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)")
![Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Flow-Cytometry-NBP2-89443-img0001.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] - Immunocytochemical labeling of ROBO1 in mouse C2C12. The cells were labeled with rabbit polyclonal ROBO1 (C-terminal region) in the absence or presence of blocking peptide. The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23373_rabbit-robo1-pab-19920231856944.jpg "Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] - Immunocytochemical labeling of ROBO1 in mouse C2C12. The cells were labeled with rabbit polyclonal ROBO1 (C-terminal region) in the absence or presence of blocking peptide. The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: ROBO1 Antibody [NBP3-23373] - Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-ROBO1 (C-terminal region) in absence (lanes 1-3) or presence of ROBO1 (C-terminal region) blocking peptide (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23373_rabbit-robo1-pab-199202317594418.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit MUC1 mAb (Mc5) - Chimeric [NBP3-21451] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 at 10 ug/ml for1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/antibody/nbp3-21451_rabbit-muc1-mab-mc5-chimeric-immunocytochemistry-immunofluorescence-1212202413438.png "Immunocytochemistry/ Immunofluorescence: Rabbit MUC1 mAb (Mc5) - Chimeric [NBP3-21451] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Mc5 at 10 ug/ml for1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.")
![Western Blot: Rabbit MUC1 mAb (Mc5) - Chimeric [NBP3-21451] - MCF7 cell lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of Mc5 at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified breast epithelial mucin is 122.1kDa, but this protein is heavily glycosylated and lipidated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/antibody/nbp3-21451_rabbit-muc1-mab-mc5-chimeric-western-blot-1212202413857..png)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (SPM533) [NBP2-32878] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1/ EMA Monoclonal Antibody (SPM533).](http://images.novusbio.com/fullsize/MUC1-Antibody-SPM533-Immunohistochemistry-Paraffin-NBP2-32878-img0001.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (SPM533) [NBP2-32878] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1/ EMA Monoclonal Antibody (SPM533).")
![Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883] - Human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)](http://images.novusbio.com/fullsize/Involucrin-Antibody-IVRN-827-Immunohistochemistry-Paraffin-NBP2-44883-img0001.jpg "Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883] - Human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)")