| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47883-0.1mg | |||
| IHC-Prediluted | NBP2-48359 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33174 | |||
| IHC-Prediluted | NBP2-44661 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54385-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44335 | |||
| Azide and BSA Free | NBP2-34781-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26680-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62561-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62565-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37900-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54667-25ul | |||
![Western Blot: MUC1 Antibody (HMPV) [NBP2-44657] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 Antibody (HMPV).](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Western-Blot-NBP2-44657-img0005.jpg "Western Blot: MUC1 Antibody (HMPV) [NBP2-44657] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 Antibody (HMPV).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMPV) [NBP2-44657] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (HMPV).](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Immunohistochemistry-Paraffin-NBP2-44657-img0001.jpg)
![Flow Cytometry: MUC1 Antibody (HMPV) [NBP2-44657] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells. MUC1 Antibody (HMPV) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)](http://images.novusbio.com/fullsize/MUC1-Antibody-HMPV-Flow-Cytometry-NBP2-44657-img0003.jpg)
![Western Blot: MUC1 Antibody (GP1.4) [NBP2-29408] - Western Blot Analysis of MCF-7 cell lysate using MUC1 Mouse Monoclonal Antibody (GP1.4).](http://images.novusbio.com/fullsize/MUC1-Antibody-GP1-4-Western-Blot-NBP2-29408-img0005.jpg "Western Blot: MUC1 Antibody (GP1.4) [NBP2-29408] - Western Blot Analysis of MCF-7 cell lysate using MUC1 Mouse Monoclonal Antibody (GP1.4).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (GP1.4) [NBP2-29408] - Formalin-paraffin human breast cancer stained with EMA Ab (GP1.4). Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/MUC1-Antibody-GP1-4-Immunohistochemistry-Paraffin-NBP2-29408-img0002.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31/1395R).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Western-Blot-NBP2-53340-img0005.jpg "Western Blot: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31/1395R).")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31/1395R).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Immunohistochemistry-Paraffin-NBP2-53340-img0003.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31/PECAM-1 Antibody (C31/1395R).followed by goat anti-rabbit- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Flow-Cytometry-NBP2-53340-img0002.jpg)
![Protein Array: CD31/PECAM-1 Antibody (C31/1395R) [NBP2-53340] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31/PECAM-1 Antibody (C31/1395R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-1395R-Protein-Array-NBP2-53340-img0004.jpg)
![Western Blot: CD31/PECAM-1 Antibody (SPM532) [NBP2-32818] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (SPM532)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM532-Western-Blot-NBP2-32818-img0003.jpg "Western Blot: CD31/PECAM-1 Antibody (SPM532) [NBP2-32818] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (SPM532)")
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (SPM532) [NBP2-32818] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with CD31/PECAM-1 Antibody (SPM532) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM532-Immunocytochemistry-Immunofluorescence-NBP2-32818-img0002.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (SPM532) [NBP2-32818] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31/PECAM-1 Antibody (SPM532).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-SPM532-Immunohistochemistry-Paraffin-NBP2-32818-img0001.jpg)
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-2622024152247.jpg "Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.")
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-262202415253312.jpg)
![Western Blot: CD44 Antibody (19H12) [NBP3-26680] - Positive Western Blot detected in: A549 whole cell lysate, MCF7 whole cell lysate, HepG2 whole cell lysate. All lanes: CD44 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa Observed band size: 81 kDa](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241136303.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (19H12) [NBP3-26680] - Staining of Hela with CD44 Antibody (19H12) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 515-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241323630.jpg)
![Flow Cytometry: CD44 Antibody (19H12) [NBP3-26680] - Overlay Peak curve showing Hela cells stained with CD44 Antibody (19H12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-282202414312756.jpg)
![Western Blot: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Western Blot using anti-MUC1 antibody SM3. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of SM3 (NBP2-62561) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62561 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Western-Blot-NBP2-62561-img0002.jpg "Western Blot: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Western Blot using anti-MUC1 antibody SM3. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of SM3 (NBP2-62561) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62561 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. SM3 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of SM3 (NBP2-62561). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62561 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of type II pneumocytes may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Immunohistochemistry-NBP2-62561-img0001.jpg)
![Flow Cytometry: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Flow-cytometry using the anti-MUC1 SM3. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of SM3 (NBP2-62561, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Flow-Cytometry-NBP2-62561-img0003.jpg)
![Immunofluorescence: MUC1 Antibody (SM3) - Chimeric [NBP2-62561] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody SM3. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of SM3 (NBP2-62561) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing clear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom; NBP2-62561, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-Chimeric-Immunofluorescence-NBP2-62561-img0004.jpg)
![Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Western-Blot-NBP2-62565-img0001.jpg "Western Blot: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Western Blot using anti-MUC1 antibody HMFG2. MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of HMFG2 (NBP2-62565) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa, though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. NBP2-62565 successfully detected human MUC1 in MCF-7 breast cancer cells.")
![Immunohistochemistry-Paraffin: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunohistochemical staining of human lung tissue using anti-MUC1 antibody. HMFG2 Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rabbit-chimeric version of HMFG2 (NBP2-62565). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62565 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong staining of type II pneumocytes may be observed. Recommended concentration, 1-2 ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunohistochemistry-Paraffin-NBP2-62565-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Flow-cytometry using the anti-MUC1 antibody HMFG2. MCF-7 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of HMFG2 (NBP2-62565, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Flow-Cytometry-NBP2-62565-img0002.jpg)
![Immunofluorescence: MUC1 Antibody (HMFG2) - Chimeric [NBP2-62565] - Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody HMFG2. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed MCF-7 cells on Shi-fix coverslips stained with the chimeric rabbit version of HMFG2 (NBP2-62565) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62565, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-HMFG2-Chimeric-Immunofluorescence-NBP2-62565-img0004.jpg)
![Recombinant Human MUC-1 Protein [H00004582-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-MUC-1-Protein-H00004582-Q01-img0001.jpg "Recombinant Human MUC-1 Protein [H00004582-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: GRB7 Antibody (JE50-05) [NBP3-32404] - Western blot analysis of GRB7 on different lysates with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/1,000 dilution. Lane 1: A431 cell lysate (40 ug/Lane) Lane 2: HepG2 cell lysate (40 ug/Lane) Lane 3: SK-Br-3 cell lysate (20 ug/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-27820241521241.jpg "Western Blot: GRB7 Antibody (JE50-05) [NBP3-32404] - Western blot analysis of GRB7 on different lysates with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/1,000 dilution. Lane 1: A431 cell lysate (40 ug/Lane) Lane 2: HepG2 cell lysate (40 ug/Lane) Lane 3: SK-Br-3 cell lysate (20 ug/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRB7 Antibody (JE50-05) [NBP3-32404] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32404) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29820241420977.jpg)
![Immunocytochemistry/ Immunofluorescence: GRB7 Antibody (JE50-05) [NBP3-32404] - Immunocytochemistry analysis of SK-Br-3 cells labeling GRB7 with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB7 antibody (NBP3-32404) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29202410484861.jpg)
![Flow Cytometry: GRB7 Antibody (JE50-05) [NBP3-32404] - Flow cytometric analysis of SK-Br-3 cells labeling GRB7. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32404, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32404_rabbit-grb7-mab-je50-05-29202416453299.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NBP1-84007-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0011.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human colorectal cancer shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0009.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human skin shows weak to moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0010.jpg)
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0003.jpg)
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0005.jpg)
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0007.jpg)
![Western Blot: MMP-2 Antibody [NBP2-54667] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-54667-img0003.jpg "Western Blot: MMP-2 Antibody [NBP2-54667] - Analysis in human cell line U-87 MG.")
![Immunohistochemistry-Paraffin: MMP-2 Antibody [NBP2-54667] - Staining of human urinary bladder shows weak to moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Immunohistochemistry-Paraffin-NBP2-54667-img0007.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody [NBP2-54667] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Immunohistochemistry-Paraffin-NBP2-54667-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody [NBP2-54667] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Immunohistochemistry-Paraffin-NBP2-54667-img0005.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody [NBP2-54667] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Immunohistochemistry-Paraffin-NBP2-54667-img0006.jpg)