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![Flow Cytometry: VE-Cadherin Antibody (BV13) [NBP1-43348] - Analysis using the Biotin conjugate of NBP1-43348. Staining of bEnd.3 cell line with 0.5 micrograms of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.125 micrograms of Anti-Mouse CD144 (VE-Cadherin) Biotin purple histogram) followed by Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV13-Flow-Cytometry-NBP1-43348-img0003.jpg "Flow Cytometry: VE-Cadherin Antibody (BV13) [NBP1-43348] - Analysis using the Biotin conjugate of NBP1-43348. Staining of bEnd.3 cell line with 0.5 micrograms of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.125 micrograms of Anti-Mouse CD144 (VE-Cadherin) Biotin purple histogram) followed by Streptavidin PE. Total viable cells were used for analysis.")
![Flow Cytometry: VE-Cadherin Antibody (BV13) [NBP1-43348] - Staining of bEnd.3 cell line with 0.5 ug of Rat IgG1 K Isotype Control Purified (open histogram) or 0.5 ug of AntiMouse CD144 (VE-Cadherin) Purified (filled histogram) followed by F(ab')2 Anti-Rat IgG PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV13-Flow-Cytometry-NBP1-43348-img0001.jpg)
![Western Blot: Periostin Antibody [NBP1-59151] - Titration: 1 ug/ml Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize/Periostin-Antibody-Western-Blot-NBP1-59151-img0005.jpg "Western Blot: Periostin Antibody [NBP1-59151] - Titration: 1 ug/ml Positive Control: 721_B cell lysate.")
![Immunohistochemistry-Paraffin: Periostin Antibody [NBP1-59151] - Human small intestine tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/Periostin-Antibody-Immunohistochemistry-Paraffin-NBP1-59151-img0006.jpg)
![Immunohistochemistry-Paraffin: Periostin Antibody [NBP1-59151] - Human uterus.](http://images.novusbio.com/fullsize/Periostin-Antibody-Immunohistochemistry-Paraffin-NBP1-59151-img0003.jpg)
![Western Blot: D-Dimer Antibody (DD1) [NB110-8374] - D-dimer, D-monomer, and fibrinogen immunodetection by MAb DD1 in Western blotting. Lane 1: D-dimer Lane 2: D-monomer Lane 3: Fibrinogen Lane 4: D-dimer treated with mercaptoethanol](http://images.novusbio.com/fullsize/D-Dimer-Antibody-DD1-Western-Blot-NB110-8374-img0004.jpg "Western Blot: D-Dimer Antibody (DD1) [NB110-8374] - D-dimer, D-monomer, and fibrinogen immunodetection by MAb DD1 in Western blotting. Lane 1: D-dimer Lane 2: D-monomer Lane 3: Fibrinogen Lane 4: D-dimer treated with mercaptoethanol")
![Immunoassay: D-Dimer Antibody (DD1) [NB110-8374] - Titration curves of D-dimer specific monoclonal antibody DD1. Antigen for coating: 200 ng/well ( - D-dimer, - fibrinogen, - D-monomer).](http://images.novusbio.com/fullsize/D-Dimer-Antibody-DD1-Immunoassay-NB110-8374-img0003.jpg)
![Western Blot: MMP-19 Antibody [NBP1-57092] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/MMP-19-Antibody-Western-Blot-NBP1-57092-img0001.jpg "Western Blot: MMP-19 Antibody [NBP1-57092] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57092] - Human urinary bladder tissue, Ganglionic cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57092-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57092] - Control-Human small intestine, Sample-Human colorectal cancer, antibody concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57092-img0002.jpg)
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57092] - Human urinary bladder tissue, Ganglionic cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57092-img0003.jpg)
![Western Blot: MMP-19 Antibody [NBP1-57094] - HepG2 cell lysate, Antibody Titration: 5.0-10.0ug/ml](http://images.novusbio.com/fullsize/MMP-19-Antibody-Western-Blot-NBP1-57094-img0009.jpg "Western Blot: MMP-19 Antibody [NBP1-57094] - HepG2 cell lysate, Antibody Titration: 5.0-10.0ug/ml")
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57094] - Human small intestine, Sample-Human colorectal cancer](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57094-img0010.jpg)
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57094] - Human urinary bladder tissue, Ganglionic cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57094-img0007.jpg)
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP1-57094] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP1-57094-img0008.jpg)
![Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Osteocalcin-Antibody-2T1J7-Western-Blot-NBP3-16081-img0001.jpg "Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Osteocalcin-Antibody-2T1J7-Western-Blot-NBP3-16081-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16081_rabbit-osteocalcin-mab-2t1j7-5220251138912.jpg)
![Flow Cytometry: CD44 Antibody (5035-41.1D) [NB100-65905] - Analysis using the FITC conjugate of NB100-65905. Staining of mouse spleen cells.](http://images.novusbio.com/fullsize/CD44-Antibody-5035-41-1D-Flow-Cytometry-NB100-65905-img0002.jpg "Flow Cytometry: CD44 Antibody (5035-41.1D) [NB100-65905] - Analysis using the FITC conjugate of NB100-65905. Staining of mouse spleen cells.")
![Flow Cytometry: CD44 Antibody (5035-41.1D) [NB100-65905] - Mouse spleen cells.](http://images.novusbio.com/fullsize/CD44-Antibody-5035-41-1D-Flow-Cytometry-NB100-65905-img0001.jpg)
![Western Blot:GRB7 Antibody (OTI15E7)[NBP3-44931]](http://images.novusbio.com/fullsize/nbp3-44864_mouse-tyrosine-hydroxylase-mab-oti1a4-immunofluorescence-1812026166266.jpg "Western Blot:GRB7 Antibody (OTI15E7)[NBP3-44931]")
![Western Blot:GRB7 Antibody (OTI15E7)[NBP3-44931]](http://images.novusbio.com/fullsize/nbp3-44931_mouse-grb7-mab-oti15e7-western-blot-18120261618180.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-29651-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.")
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP2-29651] - Collagen I Antibody [NBP2-29651] - fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-NBP2-29651-img0004.jpg)
![Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-278202415212464.jpg "Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Immunocytochemistry analysis of MCF7 cells labeling beta-Catenin with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-29202410334026.jpg)
![Flow Cytometry: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Flow cytometric analysis of MCF7 cells labeling beta-Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32079, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-392024884635.jpg)