| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47804-0.1mg | |||
| IHC-Prediluted | NBP2-48176 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48420 | |||
| Azide and BSA Free | NBP3-11454 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34749-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-65535-0.025mg | |||
![Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-278202415295585.jpg "Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403] - Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202410334095.jpg)
![Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403] - Flow cytometric analysis of MCF7 cells labeling GRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32403, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202416372159.jpg)
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-20720231348119.jpg%3Fv=1681426096 "Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).")
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-19920231410116.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-119202316331610.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).")
![Western Blot: beta-Actin Antibody [NBP3-23319] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-19920231915417.jpg)
![Western Blot: Collagen I Native Protein [NBP1-97266] - Western blot of Human Collagen I. Lane 1: Human Collagen 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen I antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed molecular weight: 139 & 130 kDa, 139 & 130 kDa for Collagen I. Other Band(s): Collagen I splice variants and isoforms.](http://images.novusbio.com/fullsize/Collagen-I-Native-Protein-Western-Blot-NBP1-97266-img0002.jpg "Western Blot: Collagen I Native Protein [NBP1-97266] - Western blot of Human Collagen I. Lane 1: Human Collagen 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen I antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed molecular weight: 139 & 130 kDa, 139 & 130 kDa for Collagen I. Other Band(s): Collagen I splice variants and isoforms.")
![SDS-Page: Collagen I Native Protein [NBP1-97266] - SDS-PAGE Results of Human Collagen I. Lane 1: Human Collagen I Reduced (5.0ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human Collagen I Non-Reduced (5.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type I trimer, Type I dimer a1, a2, Type I monomer a1, a2.](http://images.novusbio.com/fullsize/Collagen I Native Protein-SDS-Page-NBP1-97266-img0005.jpg)
![Western Blot: FLRT1 Antibody [NBP1-31403] - Sample (30ug whole cell lysate) A: H1299B: HeLa S3 7.5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Western-Blot-NBP1-31403-img0005.jpg "Western Blot: FLRT1 Antibody [NBP1-31403] - Sample (30ug whole cell lysate) A: H1299B: HeLa S3 7.5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: FLRT1 Antibody [NBP1-31403] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31403-img0003.jpg)
![Immunohistochemistry-Paraffin: FLRT1 Antibody [NBP1-31403] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/FLRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31403-img0004.jpg)
![Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ECM-1/Secretory Component P85 Mouse Monoclonal Antibody (ECM1/792).](http://images.novusbio.com/fullsize/ECM-1-Secretory-Component-P85-Antibody-rECM1-792-Immunohistochemistry-Paraffin-NBP2-44397-img0002.jpg "Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ECM-1/Secretory Component P85 Mouse Monoclonal Antibody (ECM1/792).")
![Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).](http://images.novusbio.com/fullsize/ECM-1-Secretory-Component-P85-Antibody-rECM1-792-Immunohistochemistry-Paraffin-NBP2-44397-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (DF1485) [NBP2-44653] - Confocal immunofluorescence image HeLa cells using labeled Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/CD44-Antibody-DF1485-Immunofluorescence-NBP2-44653-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (DF1485) [NBP2-44653] - Confocal immunofluorescence image HeLa cells using labeled Green (CF488) and Red Dot is used to label the nuclei Red.")
![Immunohistochemistry-Paraffin: CD44 Antibody (DF1485) [NBP2-44653] - Formalin-fixed, paraffin-embedded human Breast tissue stained with CD44 Antibody (DF1485).](http://images.novusbio.com/fullsize/CD44-Antibody-DF1485-Immunohistochemistry-Paraffin-NBP2-44653-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM246).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-SPM246-Immunohistochemistry-Paraffin-NBP2-32850-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM246) [NBP2-32850] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM246).")
![Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP2-17311] - Human hepatoma. MMP19 antibody dilution: 1:500. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MMP-19-Antibody-Immunohistochemistry-Paraffin-NBP2-17311-img0001.jpg "Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP2-17311] - Human hepatoma. MMP19 antibody dilution: 1:500. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Native-Protein-Western-Blot-NBP1-97267-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.")
![SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced [1.0ug]. 4-20% Gel Coomassie Stained. Observed: alpha1 bands.](http://images.novusbio.com/fullsize/Collagen III alpha 1-COL3A1 Native Protein-SDS-Page-NBP1-97267-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-212024939358.jpg "Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-212024943552.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25412_rabbit-claudin-3-mab-hl1527-azide-and-bsa-free-western-blot-2611202518255013.jpg)
![Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of representative areas of synovial tissue samples taken from patients with end-stage osteoarthritis (OA) or refractory rheumatoid arthritis (RA) is shown. Expression pattern and staining intensity of RA samples represent the stronger intensity of synovial inflammation when compared with OA samples.](http://images.novusbio.com/fullsize/CD44-Antibody-VFF-17-variant-7-8-Immunohistochemistry-NB100-65535-img0003.jpg "Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of representative areas of synovial tissue samples taken from patients with end-stage osteoarthritis (OA) or refractory rheumatoid arthritis (RA) is shown. Expression pattern and staining intensity of RA samples represent the stronger intensity of synovial inflammation when compared with OA samples.")
![Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of osteoarthritis. Phenotypic markers that are instrumental in extracellular matrix alteration are detectable only at a low level (MMP-1, CD44v7/8) in areas that are slightly FAP-positive. FAP, fibroblast activation protein; MMP, matrix metalloproteinase.](http://images.novusbio.com/fullsize/CD44-Antibody-VFF-17-variant-7-8-Immunohistochemistry-NB100-65535-img0001.jpg)
![Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of rheumatoid arthritis. Expression of fibroblast activation protein (FAP) is accompanied by accumulation of activation markers (MMP-1 and MMP-13) and splice variants of CD44 (v3 and v7/8) that are known to correlate with the degree of inflammation and that are involved in extracellular matrix deposition.](http://images.novusbio.com/fullsize/CD44-Antibody-VFF-17-variant-7-8-Immunohistochemistry-NB100-65535-img0002.jpg)