| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35691-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92909-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37384-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52512-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47885-0.1mg | |||
| IHC-Prediluted | NBP2-48361 | |||
![Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Western-Blot-NBP3-15841-img0007.jpg "Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] - Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15841_rabbit-versican-mab-6d8k9-72202512443356.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Mouse brain using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0003.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Rat kidney using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0001.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Human esophageal cancer using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0002.jpg)
![Immunohistochemistry: Fibrillin 1 Antibody [NBP3-35691] - Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35691_rabbit-fibrillin-1-pab-1012202411404052.jpg "Immunohistochemistry: Fibrillin 1 Antibody [NBP3-35691] - Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Fibrillin 1 Antibody [NBP3-35691] - Immunofluorescence analysis of NIH-3T3 cells using Fibrillin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35691_rabbit-fibrillin-1-pab-1012202411392987.jpg)
![Western Blot: Fibrillin 1 Antibody [NBP3-35691] - Western blot analysis of lysates from Rat pancreas, using Fibrillin 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35691_rabbit-fibrillin-1-pab-1012202411364683.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillin 1 Antibody [NBP3-35691] - Immunofluorescence analysis of C6 cells using Fibrillin 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35691_rabbit-fibrillin-1-pab-1012202411381274.jpg)
![Immunohistochemistry: Fibrillin 1 Antibody [NBP3-35691] - Immunohistochemistry analysis of paraffin-embedded Human liver using Fibrillin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35691_rabbit-fibrillin-1-pab-1012202411353066.jpg)
![Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Western-Blot-NBP2-92909-img0005.jpg "Western Blot: ACTL6B Antibody [NBP2-92909] - Analysis of extracts of various cell lines, using ACTL6B at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.")
![Immunocytochemistry/Immunofluorescence: ACTL6B Antibody [NBP2-92909] - Analysis of A-549 cells using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92909-img0001.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded mouse jejunum using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0004.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney cancer using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0002.jpg)
![Immunohistochemistry-Paraffin: ACTL6B Antibody [NBP2-92909] - Paraffin-embedded human kidney using ACTL6B .](http://images.novusbio.com/fullsize/ACTL6B-Antibody-Immunohistochemistry-Paraffin-NBP2-92909-img0003.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0002.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0001.jpg)
![Immunoprecipitation: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Collagen III alpha 1/COL3A1 antibody (NBP3-15309). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunoprecipitation-NBP3-15309-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [Collagen III alpha 1/COL3A1] - Confocal imaging of HeLa cells using Collagen III alpha 1/COL3A1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15309_rabbit-collagen-iii-alpha-1-col3a1-mab-6y9c6-52202517273527.jpg)
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0005.jpg "Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Mouse heart, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0004.jpg)
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of HepG2 cells, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of NIH/3T3 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025126533.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of Hep G2 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125497.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of C6 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125425.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Western Blot: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Western blot analysis using ITGA2B mAb against HEK293 (1) and ITGA2B (AA: 45-149)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-Western-Blot-NBP2-37384-img0003.jpg "Western Blot: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Western blot analysis using ITGA2B mAb against HEK293 (1) and ITGA2B (AA: 45-149)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ITGA2B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-Immunohistochemistry-NBP2-37384-img0006.jpg)
![Flow Cytometry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Flow cytometric analysis of HL60 cells using ITGA2B mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-Flow-Cytometry-NBP2-37384-img0004.jpg)
![Western Blot: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Western blot analysis using ITGA2B mAb against human ITGA2B recombinant protein. (Expected MW is 36.9 kDa)](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-Western-Blot-NBP2-37384-img0002.jpg)
![Immunohistochemistry: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ITGA2B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-Immunohistochemistry-NBP2-37384-img0005.jpg)
![ELISA: Integrin alpha 2b/CD41 Antibody (2E10C9) [NBP2-37384] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Integrin-alpha-2b-CD41-Antibody-2E10C9-ELISA-NBP2-37384-img0001.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0004.jpg "Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)")
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of bladder cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-Paraffin-NBP2-52512-img0003.jpg)
![Flow Cytometry: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Flow-Cytometry-NBP2-52512-img0001.jpg)
![Western Blot: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Western-Blot-NBP2-52512-img0005.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Analysis of ovarian cancer tissues using MMP14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-Immunohistochemistry-NBP2-52512-img0002.jpg)
![ELISA: MMP-14/MT1-MMP Antibody (6A11D1) [NBP2-52512] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-6A11D1-ELISA-NBP2-52512-img0006.jpg)
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.](http://images.novusbio.com/fullsize/RPSA-Antibody-RP-01-Immunocytochemistry-Immunofluorescence-NBP3-09109-img0001.jpg "Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.")
![Western Blot: Mouse Monoclonal RPSA Antibody (RP-01) [NBP3-09109] - Western blotting analysis was performed on urea buffer extracts of Ramos, Raji, HeLa, Jurkat, and Huvec cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody RP-01 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.RPSA was detected at ~37 kDa in all tested cell lines.](http://images.novusbio.com/fullsize/antibody/nbp3-09109_mouse-monoclonal-rpsa-antibody-rp-01-sandwich-elisa-151202513658..jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Western-Blot-H00000059-M02-img0010.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Western blot analysis of alpha-Smooth Muscle Actin expression in human omentum, serous carcinoma, blotted with alpha-Smooth Muscle Actin Antibody (4A8-2H3). The band indicates an observed molecular weight of 42 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Immunofluorescence analysis of alpha-Smooth Muscle Actin on HeLa cell. Antibody concentration 1 - 10 ug/ml.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-Immunocytochemistry-Immunofluorescence-H00000059-M02-img0008.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A8-2H3) [H00000059-M02] - Detection limit for recombinant GST tagged alpha-Smooth Muscle Actin Antibody is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A8-2H3-ELISA-H00000059-M02-img0011.jpg)
![Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Western-Blot-NBP2-44659-img0004.jpg "Western Blot: MUC1 Antibody (MUC1/967) [NBP2-44659] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).")
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Breast Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) [NBP2-44659] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).](http://images.novusbio.com/fullsize/MUC1-Antibody-MUC1-967-Immunohistochemistry-Paraffin-NBP2-44659-img0003.jpg)