| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46817-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-11660 | |||
| DyLight 680 | NBP3-11664 | |||
| DyLight 800 | NBP3-11663 | |||
| FITC | NBP3-11662 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 680 | NBP3-11667 | |||
| DyLight 800 | NBP3-11666 | |||
| FITC | NBP3-11665 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37825-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37823-0.025mg | |||
![Western Blot: GSTA1 Antibody [NBP2-46817] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Western-Blot-NBP2-46817-img0003.jpg "Western Blot: GSTA1 Antibody [NBP2-46817] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG, Lane 4: Human plasma, Lane 5: Human Analysis of human liver tissue.")
![Immunocytochemistry/Immunofluorescence: GSTA1 Antibody [NBP2-46817] - Staining of human cell line Hep G2 shows localization to cytosol.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-46817-img0004.jpg)
![Immunohistochemistry: GSTA1 Antibody [NBP2-46817] - Staining of human Analysis of human liver tissue. shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/GSTA1-Antibody-Immunohistochemistry-NBP2-46817-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0004.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. Fusion nuclear protein (red) stained with purified DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] (detection by Goat anti-mouse IgG1 Alexa Fluor 594).")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554] - Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK Epitope Tag Antibody (F-tag-01) [NB500-554]. Merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-F-tag-01-Immunocytochemistry-Immunofluorescence-NB500-554-img0003.jpg)
![Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/N-OLLAS-Vector-Western-Blot-NBA1-07083-img0002.jpg "Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![N OLLAS Vector [NBA1-07083] - Vector map of pCMV-N OLLAS](http://images.novusbio.com/fullsize/N-OLLAS-Vector-NBA1-07083-img0003.jpg)
![Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07085-img0001.jpg "Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Vector Map of pCMV-SD OLLAS](http://images.novusbio.com/fullsize/SD-OLLAS-DYKDDDDK-Vector-NBA1-07085-img0002.jpg)
![Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-Western-Blot-NBP1-42781-img0020.jpg "Western Blot: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100 ng Purified histidine-tagged recombinant protein. Lane 2: 200 ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100 ng Purified GST-tagged recombinant protein. Lane 4: 100 ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.")
![ELISA: His Tag Antibody (33D10.D2.G8) [NBP1-42781] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100 ug/mL. Dilution series: 2-fold. Mid-point concentration: 200 ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB.](http://images.novusbio.com/fullsize/His-Tag-Antibody-33D10-D2-G8-ELISA-NBP1-42781-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: HSV Tag Antibody (223) [NBP2-43816] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hsv Tag stained by Hsv Tag antibody [223] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/HSV-Tag-Antibody-223-Immunocytochemistry-Immunofluorescence-NBP2-43816-img0002.jpg "Immunocytochemistry/Immunofluorescence: HSV Tag Antibody (223) [NBP2-43816] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hsv Tag stained by Hsv Tag antibody [223] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSV tag antibody [GT223] (NBP2-43816) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43816_mouse-monoclonal-hsv-tag-antibody-223-western-blot-2611202518272324.jpg)
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunocytochemistry-Immunofluorescence-NBP2-43626-img0010.jpg "Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunoprecipitation-NBP2-43626-img0002.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] (NBP2-43626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43626_mouse-monoclonal-v5-epitope-tag-antibody-1071-western-blot-2611202518493923.jpg)
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0002.jpg "Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] detects only native antibody and not denatured heavy and light chains.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0001.jpg)
![Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [HRP] [NBP3-11668] - Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Mouse Pure-Blot anti-Rabbit IgG Secondary antibody (eB182) [HRP].](http://images.novusbio.com/fullsize/Mouse-Pure-Blot-anti-Rabbit-IgG-Secondary-Antibody-eB182-HRP-Western-Blot-NBP3-11668-img0001.jpg "Western Blot: Mouse Pure-Blot anti-Rabbit IgG (H+L) Secondary Antibody (eB182) [HRP] [NBP3-11668] - Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Mouse Pure-Blot anti-Rabbit IgG Secondary antibody (eB182) [HRP].")
![Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunoprecipitation-NBP3-14726-img0001.jpg "Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.")
![Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0003.jpg "Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.")
![Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0004.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0011.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 min at RT, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 mins at RT, then probed with a DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] at 1:500 for 1 hr at RT. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at 1:2000 for 1 hr at RT. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of 30ug of whole cell lysates from HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under the control of a Tetracycline promoter (Tet), and either left untreated (-) or treated with anhydrotetracycline (+), onto an SDS-PAGE gel, using [NBP2-37823]. Observed molecular weight ~30 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA in HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under control of a Tetracycline (Tet) promoter, untreated (-) or treated with anhydrotetracycline (+). Complexes formed by incubating 1ml of HeLa cell lysate with 3ug of [NBP2-37823] overnight at 4C. Immune complexes were captured on 20ul Protein G agarose and eluted by boiling in Laemmli sample buffer. Samples were resolved on a 10% Bis-Tris acrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hr at RT. Membrane was probed with a rabbit anti-FLAG pAb at 1:200 in 3% BSA in TBST overnight at 4C, washed in TBST, and incubated with an HRP-conjugated donkey anti-rabbit IgG secondary Ab at 1:15000 in 1% milk in TBST for 1 hr at RT. Chemiluminescence performed using SuperSignal West Dura. Data courtesy of Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0010.jpg)