DYKDDDDK Epitope Tag Antibody (FG4R) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ICC/IF
Clone
FG4R
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594

DYKDDDDK Epitope Tag Antibody (FG4R) [Alexa Fluor® 594] Summary

Immunogen
Synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity
Detects Flag tag from various samples. has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DYKDDDDK Epitope Tag Antibody (FG4R) [Alexa Fluor® 594]

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DYKDDDDK Epitope Tag Antibody (NBP2-37823AF594) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP2-37823AF594). (Showing 1 - 5 of 5 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-37823AF350
Alexa Fluor 405 NBP2-37823AF405
Alexa Fluor 488 NBP2-37823AF488
Alexa Fluor 532 NBP2-37823AF532
Alexa Fluor 594 NBP2-37823AF594
Alexa Fluor 647 NBP2-37823AF647
Alexa Fluor 700 NBP2-37823AF700
Alexa Fluor 750 NBP2-37823AF750
Biotin NBP2-37823B
DyLight 350 NBP2-37823UV
DyLight 405 NBP2-37823V
DyLight 488 NBP2-37823G
DyLight 550 NBP2-37823R
DyLight 594 NBP2-37823DL594
DyLight 650 NBP2-37823C
DyLight 680 NBP2-37823FR
DyLight 755 NBP2-37823IR
FITC NBP2-37823F
HRP NBP2-37823H
Janelia Fluor 549 NBP2-37823JF549
Janelia Fluor 646 NBP2-37823JF646

Additional DYKDDDDK Epitope Tag Products

Array NBP2-37823AF594

Blogs on DYKDDDDK Epitope Tag.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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