SD OLLAS/DYKDDDDK Vector

Western Blot: SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SD OLLAS/DYKDDDDK Vector [NBA1-07085] - Vector Map of pCMV-SD OLLAS

• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

SD OLLAS/DYKDDDDK Vector Summary

• Specificity: pCMV-SD OLLAS / DYKDDDDK Vector for the expression of secretory protein with N-terminally FLAG(DYKDDDDK) and OLLAS dual tandem tagged.

Applications/Dilutions

• Application Notes: Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 5 mM Tris pH 8.0
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for SD OLLAS/DYKDDDDK Vector

• DDDDK vector
• DYKDDDDK epitope tag vector
• DYKDDDDK vector
• ECS tag vector
• ECS vector
• Enterokinase Cleavage Site epitope tag vector
• Enterokinase Cleavage Site tag vector
• FLAG vector (FLAG is a trademark of Sigma Aldrich)
• OLLA-2 vector
• xxxDDDDK epitope tag vector

Background

The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be secreted from the cell due to the inclusion of a signal peptide. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.