| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-62005B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25949 | |||
.png "cMYC was detected in immersion fixed paraffin-embedded sections of human B-cell Lymphoma using Rabbit Anti-Human cMYC, Monoclonal Antibody (Catalog # NBP3-20492) at 5ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=")
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder." alt="cMYC was detected in immersion fixed paraffin-embedded sections of human B-cell Lymphoma using Rabbit Anti-Human cMYC, Monoclonal Antibody (Catalog # NBP3-20492) at 5ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20789 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96893 | |||
| FITC | NBP1-96908 | |||
| HRP | NBP1-96874 | |||
| Rhodamine | NBP1-96909 | |||
| Texas Red | NBP1-96757 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48089 | |||
| Azide and BSA Free | NBP2-86682 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81037-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76651-0.025ml | |||
![Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Western-Blot-NB600-325-img0002.jpg "Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).")
![Western Blot: IgG Antibody (RM104) [NBP2-62005] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20 ng/lane), using NBP2-62005 at 0.2ug/ml. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.](http://images.novusbio.com/fullsize/IgG-Antibody-RM104-Unconjugated-Western-Blot-NBP2-62005-img0004.jpg "Western Blot: IgG Antibody (RM104) [NBP2-62005] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20 ng/lane), using NBP2-62005 at 0.2ug/ml. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE.")
![ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] - ELISA of mouse immunoglobulins shows RM104 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62005_rabbit-monoclonal-igg-antibody-rm104-elisa-18920252340354.png)
![ELISA: IgG Antibody (RM104) - Azide and BSA Free [NBP2-62005] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62005_rabbit-monoclonal-igg-antibody-rm104-elisa-18920252339522.png)
![Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-Western-Blot-NBA1-07084-img0003.jpg "Western Blot: ND OLLAS/DYKDDDDK Vector [NBA1-07084] - Western blot comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies anti-OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2* monoclonal antibody from Sigma-Aldrich. *FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.")
![ND OLLAS/DYKDDDDK Vector [NBA1-07084] - NBA1-07084: Vector Map of pCMV-ND OLLAS](http://images.novusbio.com/fullsize/ND-OLLAS-DYKDDDDK-Vector-NBA1-07084-img0004.jpg)
![Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-Western-Blot-NBP1-97370-img0006.jpg "Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.")
![Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot showing detection of Maltose Binding protein antibody - BSA Free.Lane 1: MW markers. Lane 2: Maltose Binding Protein [0.05 ug]. Protein was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS 30 min at 20C. Primary Antibody: Anti-MBP (RABBIT) antibody was used at 1:1000 overnight at 4C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at 20C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-Western-Blot-NBP1-97370-img0005.jpg)
![N/A: Maltose Binding Protein Antibody - BSA Free [NBP1-97370]](http://images.novusbio.com/fullsize/Maltose-Binding-Protein-Antibody---BSA-Free-N-A-NBP1-97370-img0004.jpg)
![Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code ) for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/nbp1-97370_rabbit-polyclonal-maltose-binding-protein-antibody-255202313135159.jpg)
![SDS-Page: Donkey IgG Isotype Control [NBP1-97048] - SDS-PAGE of Donkey IgG Isotype control. Lane 1: Donkey IgG Isotype control Reduced (1.0ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Donkey IgG Isotype control Non-Reduced (1.0ug). 4-20% Gel, Coomassie Stained.](http://images.novusbio.com/fullsize/Donkey IgG Isotype Control-SDS-Page-NBP1-97048-img0003.jpg "SDS-Page: Donkey IgG Isotype Control [NBP1-97048] - SDS-PAGE of Donkey IgG Isotype control. Lane 1: Donkey IgG Isotype control Reduced (1.0ug). Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Donkey IgG Isotype control Non-Reduced (1.0ug). 4-20% Gel, Coomassie Stained.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).](http://images.novusbio.com/fullsize/c-Myc-Antibody-SPM237-Immunohistochemistry-Paraffin-NBP2-45145-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).")
![Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - Analysis of 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 ug whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 ug of DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DYKDDDDK Epitope Tag Antibody (231) diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-231-Immunoprecipitation-NBP2-43570-img0001.jpg "Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - Analysis of 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 ug whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 ug of DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DYKDDDDK Epitope Tag Antibody (231) diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - DDDDK Tag antibody [GT231] detects DDDDK Tag protein by Western blot analysis.<br>A. 30 ug 293T whole cell extract <br>B. 30 ug whole cell extract of DDDDK-tagged protein expressing 293T cells<br>12 % SDS-PAGE<br>DDDDK Tag antibody [GT231] (NBP2-43570) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-43570_mouse-dykddddk-epitope-tag-mab-231-9102024205644.jpg)
![SDS-Page: Recombinant Firefly Luciferase Protein [NBP1-48355]](http://images.novusbio.com/fullsize/Luciferase-firefly-Protein-SDS-Page-NBP1-48355-img0001.jpg "SDS-Page: Recombinant Firefly Luciferase Protein [NBP1-48355]")
![Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-278202415212443.jpg "Western Blot: c-Myc Antibody (PS00-11) [NBP3-32201] - Western blot analysis of c-Myc on different lysates with Rabbit anti-c-Myc antibody (NBP3-32201) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Daudi cell lysate<br/> Lane 3: K562 cell lysate<br/> Lane 4: HL-60 cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 49 kDa<br/> Observed band size: 55 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32201) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: c-Myc Antibody (PS00-11) [NBP3-32201] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-c-Myc antibody (NBP3-32201) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32201) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32201_rabbit-c-myc-mab-ps00-11-298202413585552.jpg)
![Western blot analysis on different lysates with NBP3-32201 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32201 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_-western-blot-639204594550909475.jpg)
![Western blot analysis on different lysates with NBP3-32201 at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: Neuro-2a cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32201 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32201_-western-blot-639204597794751464.jpg)
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