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![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84051-img0009.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0016.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0014.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0015.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89968] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89968-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89968] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89968-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89968] - Analysis using Anti-HSPA5 antibody NBP1-89968 (A) shows similar pattern to independent antibody NBP1-89969 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89968-img0011.jpg)
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - A typical standard curve for GRP78/HSPA5 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0001.jpg "ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - A typical standard curve for GRP78/HSPA5 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - To assess parallelism, HeLa, 3T3, and C6 lysates and serum were serially diluted into assay buffer and run in the assay. The GRP78 concentration in each sample was assigned using the standard curve. Assigned concentrations were plotted as a function of sa](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0002.jpg)
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - ELISA versus Western blot analysis correlation comparison of GRP78/HSPA5 using C6 cell lysates. Lysates were serially diluted and either run in the ELISA or run on a 12% Tris-glycine gel, transferred to nitrocellulose membrane and probed for GRP78 with a](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0004.jpg)
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - HeLa cells were treated for 5 hours with 5 uM, 50 uM or 200 uM concentrations of cyclosporine A. Following the incubation, treated and untreated cells were lysed with Extraction Reagent #2 as described above. The lysates were then evaluated in the assay.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0003.jpg)