| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06019-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90198-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06333-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00348-50ul | |||
![Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 .](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-74496-img0002.jpg "Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGIF1 .")
![Immunohistochemistry: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74496-img0003.jpg)
![Western Blot: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of extracts (10ug) from 2 different cell lines.](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-74496-img0001.jpg)
![Immunohistochemistry: TGIF1 Antibody (OTI1B12) - Azide and BSA Free [NBP2-74496] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TGIF1-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74496-img0004.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Anti-MRPL11 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Western-Blot-NBP3-06019-img0001.jpg "Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Anti-MRPL11 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa")
![Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Immunochemical staining of human MRPL11 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Immunohistochemistry-Paraffin-NBP3-06019-img0003.jpg)
![Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Immunochemical staining of human MRPL11 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Immunohistochemistry-Paraffin-NBP3-06019-img0004.jpg)
![Immunoprecipitation: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - MRPL11 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-MRPL11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-MRPL11 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Immunoprecipitation-NBP3-06019-img0002.jpg)
![Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-288202412442931.jpg "Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29820241411541.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunocytochemistry analysis of HeLa cells labeling RNA Helicase A with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29202410484888.jpg)
![Western Blot: FDPS Antibody (005) [NBP2-90198] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:38 kDa](http://images.novusbio.com/fullsize/FDPS-Antibody-005-Western-Blot-NBP2-90198-img0003.jpg "Western Blot: FDPS Antibody (005) [NBP2-90198] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:38 kDa")
![<b>Biological Strategies Validation. </b>Flow Cytometry: FDPS Antibody (005) [NBP2-90198] - Analysis of Human FDPS expression on HeLa cells. The cells were treated, stained with purified anti-Human FDPS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/FDPS-Antibody-005-Flow-Cytometry-NBP2-90198-img0002.jpg)
![Immunoprecipitation: FDPS Antibody (005) [NBP2-90198] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-FDPS rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FDPS rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 38 kDa](http://images.novusbio.com/fullsize/FDPS-Antibody-005-Immunoprecipitation-NBP2-90198-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FDPS Antibody (005) [NBP2-90198] - Staining of Human FDPS in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90198 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90198_rabbit-monoclonal-fdps-antibody-005-235202512205542.png)
![Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP3-06333-img0001.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa")
![Immunoprecipitation: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody was immunoprecipitated using:Lane A: 0.5 mg Hela Whole Cell Lysate4 uL anti-EIF6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-EIF6 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 26 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunoprecipitation-NBP3-06333-img0002.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody - BSA Free [NBP3-06333] - Immunochemical staining of human integrin beta 4 binding protein in human adrenal gland with NBP3-06333 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06333_rabbit-polyclonal-integrin-beta-4-binding-protein-antibody-23520251664728.png)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody - BSA Free [NBP3-06333] - Immunochemical staining of human integrin beta 4 binding protein in human esophagus with NBP3-06333 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06333_rabbit-polyclonal-integrin-beta-4-binding-protein-antibody-23520251129341.png)
![Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-2352025162819.png "Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa")
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545641.png)
![Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-23520251664731.png)
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545651.png)
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-278202415242630.jpg "Western Blot: APE Antibody (12H1-R) [NBP3-32042] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: C6 cell lysate (20 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32042) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H1-R) [NBP3-32042] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32042) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32042) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-298202414333662.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H1-R) [NBP3-32042] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32042) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32042_mouse-ape-mab-12h1-r-2920241114842.jpg)
![Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32604) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, 10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: A431 cell lysate, 10 ug/Lane Lane 4: NIH-3T3 cell lysate, 10 ug/Lane Lane 5: Mouse testis tissue lysate, 20 ug/Lane Lane 6: Mouse heart tissue lysate, 20 ug/Lane Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 5% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32604) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-27820241631696.jpg "Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32604) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, 10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: A431 cell lysate, 10 ug/Lane Lane 4: NIH-3T3 cell lysate, 10 ug/Lane Lane 5: Mouse testis tissue lysate, 20 ug/Lane Lane 6: Mouse heart tissue lysate, 20 ug/Lane Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 5% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32604) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MSH2 antibody (NBP3-32604) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-298202414304163.jpg)
![Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunocytochemistry analysis of Daudi cells labeling MSH2 with Mouse anti-MSH2 antibody (NBP3-32604) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MSH2 antibody (NBP3-32604) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-29202410234279.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)