| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-11473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22196-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97854-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48148 | |||
| Azide and BSA Free | NBP3-11456 | |||
![Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Western-Blot-NB110-11473-img0006.jpg "Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - Immunohistochemistry analysis using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..](http://images.novusbio.com/fullsize/HSP10-EPF Antibody-Immunohistochemistry-NB110-11473-img0007.jpg)
![Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Western-Blot-NB110-11473-img0005.jpg)
![Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - analysis using Rabbit Anti-Cpn10 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..](http://images.novusbio.com/fullsize/HSP10-EPF-Antibody-Immunohistochemistry-NB110-11473-img0004.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0006.jpg "Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.")
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0003.jpg)
![Flow Cytometry: PLK1 Antibody (3C11) [NBP2-22196] - Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Flow-Cytometry-NBP2-22196-img0001.jpg)
![Western Blot: PLK1 Antibody (3C11) [NBP2-22196] - Western blot analysis using PLK1 mAb against human PLK1 recombinant protein. (Expected MW is 45.7 kDa)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Western-Blot-NBP2-22196-img0005.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (3C11) [NBP2-22196] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-Immunohistochemistry-Paraffin-NBP2-22196-img0002.jpg)
![ELISA: PLK1 Antibody (3C11) [NBP2-22196] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PLK1-Antibody-3C11-ELISA-NBP2-22196-img0004.jpg)
![Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Western-Blot-NBP2-97854-img0006.jpg "Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-97854-img0005.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-97854-img0004.jpg)
![Immunoprecipitation: NDUFB7 Antibody [NBP2-97854] - NDUFB7 was immunoprecipitated using: Lane A: 0.5 mg HL60 Whole Cell Lysate 4 ul anti-NDUFB7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFB7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunoprecipitation-NBP2-97854-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFB7 Antibody [NBP2-97854] - Immunofluorescence staining of NDUFB7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97854 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97854_rabbit-polyclonal-ndufb7-antibody-23520251129313.png)
![Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0082.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: MCM7 Antibody [NB100-252] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-Western-Blot-NB100-252-img0002.jpg)
![Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Western-Blot-NBP2-15514-img0001.jpg "Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ATP5J Antibody [NBP2-15514] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15514-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - Immunohistochemical analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATP5J-Antibody-Immunohistochemistry-Paraffin-NBP2-15514-img0002.jpg)
![Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-24764-img0002.jpg "Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-24764] - Staining of human liver tissue using an isotype control (left) and this antibody (right) at 5 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-24764-img0001.jpg)
![Western Blot: AK3L1 Antibody [NBP2-15317] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 C: HCT116 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Western-Blot-NBP2-15317-img0003.jpg "Western Blot: AK3L1 Antibody [NBP2-15317] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 C: HCT116 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - AK3L1 antibody detects AK3L1 protein at mitochondria in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. AK3L1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15317-img0004.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15317-img0001.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-15317] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15317-img0002.jpg)
![Adenylate kinase 4 antibody [N1C3-2] detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis.Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: Adenylate kinase 4 protein stained by Adenylate kinase 4 antibody [N1C3-2] (NBP2-15317) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15317_rabbit-polyclonal-ak3l1-antibody-immunocytochemistry-immunofluorescence-261120251839319.jpg)
![Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445] - Western Blot Analysis of heart tissue lysate using Cytochrome c Antibody (CTC05).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CTC05-Western-Blot-NBP2-44445-img0003.jpg "Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445] - Western Blot Analysis of heart tissue lysate using Cytochrome c Antibody (CTC05).")
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445] - Human Heart stained with Cytochrome C Monoclonal Antibody (CTC05).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CTC05-Immunohistochemistry-Paraffin-NBP2-44445-img0001.jpg)
![Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0005.jpg "Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.")
![Western Blot: CRABP2 Antibody [NBP2-15998] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE NBP2-15998 diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0001.jpg)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-15998] - Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-15998-img0002.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunohistochemistry-paraffin-261120251847573.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunocytochemistry-immunofluorescence-261120251849134.jpg)
![Western Blot: CCT5 Antibody [NBP1-31337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1N3].](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-31337-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCT5 Antibody [NBP1-31337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1N3].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-31337] - Analysis of HeLa, using NBP1-31337 at 1:200 dilution.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31337-img0002.jpg)
![Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-31337] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CCT5-Antibody-Immunohistochemistry-Paraffin-NBP1-31337-img0003.jpg)
![Western Blot: CCT5 Antibody [NBP1-31337] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CCT5-Antibody-Western-Blot-NBP1-31337-img0004.jpg)
![Immunohistochemistry: CCT5 Antibody [NBP1-31337] - Immunohistochemical analysis of frozen sections of zebrafish muscle, 5 hpf, using CCT5 antibody [N1N3] (NBP1-31337) at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp1-31337_rabbit-polyclonal-cct5-antibody-91020242013126.jpg)
![Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Western-Blot-NB100-79803-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunohistochemistry-Paraffin-NB100-79803-img0010.jpg)
![Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Human testicular seminoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunohistochemistry-Paraffin-NB100-79803-img0009.jpg)
![Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (R; 50 mcg) cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-CSE1L/CAS/Exportin-2 antibody BL1975 (Cat. No. NB100-79803) used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for WB and at 3 mcg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 3 minutes (A and B).](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunoprecipitation-NB100-79803-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of human CSE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSE1 antibody NB100-79803 (lot NB100-79803-2) used for IP at 6 ug per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody (NB100-79803-1) and another rabbit anti-CSE1 antibody. For blotting immunoprecipitated CSE1, NB100-79803 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CSE1L-CAS-Exportin-2-Antibody-Immunoprecipitation-NB100-79803-img0013.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg)
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg)