| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-302SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54545-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59632 | |||
![Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Western-Blot-NBP2-37626-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunocytochemistry-Immunofluorescence-NBP2-37626-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-Paraffin-NBP2-37626-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-NBP2-37626-img0004.jpg)
![ELISA: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-ELISA-NBP2-37626-img0001.jpg)
![Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-70875-img0002.jpg "Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.")
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0006.jpg)
![Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-70875-img0001.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0003.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0004.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0005.jpg)
![Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HADH-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70875-img0007.jpg)
![Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - NDUFS2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32639 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32639 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32639 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32639 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-2682024158268.jpg "Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - NDUFS2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32639 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32639 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32639 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32639 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds")
![Western Blot: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: K-562 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: RAW264.7 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Rat brain tissue lysate (40 ug/Lane) Lane 14: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 53 kDa Observed band size: 45 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32639) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-278202416331239.jpg)
![Immunohistochemistry: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-298202414262616.jpg)
![Immunocytochemistry/ Immunofluorescence: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Immunocytochemistry analysis of A431 cells labeling NDUFS2 with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-2920241192319.jpg)
![Flow Cytometry: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Flow cytometric analysis of A431 cells labeling NDUFS2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32639, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-392024884686.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0007.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of MCF-7 cells with ErbB2/Her2 Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0004.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Protein-Array-NBP2-33064-img0003.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-16306] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16306-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306] - Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-16306-img0006.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0003.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32515-img0004.jpg "Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunohistochemistry-Paraffin-NBP1-32515-img0007.jpg)
![Immunoprecipitation: U2AF35 Antibody [NBP1-32515] - U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody. Western blot analysis was performed using U2AF35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/U2AF35-Antibody-Immunoprecipitation-NBP1-32515-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: U2AF35 Antibody [NBP1-32515] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32515_rabbit-polyclonal-u2af35-antibody-1610202419414536.jpg)
![Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-302-img0003.jpg "Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-302-img0004.jpg)
![Western Blot: GNPDA1 Antibody [NBP2-24653] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen and 4) rat spleen using GNPDA1 antibody at 1.0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.](http://images.novusbio.com/fullsize/GNPDA1-Antibody-Western-Blot-NBP2-24653-img0001.jpg "Western Blot: GNPDA1 Antibody [NBP2-24653] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen and 4) rat spleen using GNPDA1 antibody at 1.0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24653] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/GNPDA1-Antibody-Immunohistochemistry-Paraffin-NBP2-24653-img0002.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Western-Blot-NBP2-53282-img0005.jpg "Western Blot: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).")
![Immunocytochemistry/Immunofluorescence: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Confocal Immunofluorescence image of HeLa cells using TOP2A Antibody (TOP2A/1362) Green (CF488) and Phalloidin (Red) is used to label the nuclei.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Immunocytochemistry-Immunofluorescence-NBP2-53282-img0003.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Immunohistochemistry-Paraffin-NBP2-53282-img0002.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Immunohistochemistry-Paraffin-NBP2-53282-img0001.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1362) [NBP2-53282] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Protein-Array-NBP2-53282-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0005.jpg "Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).")
![Immunohistochemistry-Paraffin: p53 Antibody (TP53/1799R) [NBP2-59631] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withp53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R)](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Immunohistochemistry-Paraffin-NBP2-59631-img0002.jpg)
![Flow Cytometry: p53 Antibody (TP53/1799R) [NBP2-59631] - Flow Cytometric Analysis of HeLa cells using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Flow-Cytometry-NBP2-59631-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0003.jpg)
![Protein Array: p53 Antibody (TP53/1799R) [NBP2-59631] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Protein-Array-NBP2-59631-img0001.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)