| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47921-0.1mg | |||
| IHC-Prediluted | NBP2-48127 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26680-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09017_rabbit-monoclonal-mhc-class-i-antibody-r1-21-2-chimeric-74202522265217.png)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP2-17053-img0003.jpg "Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/KIF22-Antibody-Immunohistochemistry-Paraffin-NBP2-17053-img0002.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF22 antibody [N3C2], Internal (NBP2-17053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17053_rabbit-polyclonal-kif22-antibody-western-blot-261120251834266.jpg)
![KIF22 antibody [N3C2], Internal detects KIF22 protein at cytoskeleton by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KIF22 protein stained by KIF22 antibody [N3C2], Internal (NBP2-17053) diluted at 1:500.Blue: Hoechst 33343 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]](http://images.novusbio.com/fullsize/nbp2-17053_rabbit-polyclonal-kif22-antibody-immunocytochemistry-immunofluorescence-2611202518283510.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0002.jpg "Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16257-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP2-16257 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-91020242021926.jpg)
![Immunohistochemistry: PA2G4/EBP1 Antibody [NBP2-16257] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PA2G4/EBP1 antibody [N3C3] (NBP2-16257) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-9102024201579.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Mantle Cell Lymphoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Immunohistochemistry-Paraffin-NBP2-44586-img0003.jpg "Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Mantle Cell Lymphoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).")
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Cyclin D1 Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Bladder Carcinoma stained with Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Immunohistochemistry-Paraffin-NBP2-44586-img0002.jpg)
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Human Cyclin D1 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Cyclin D1 Monoclonal Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0001.jpg)
![Flow Cytometry: Cyclin D1 Antibody (CCND1/809) [NBP2-44586] - Flow Cytometric analysis of human Cyclin D1 on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (CCND1/809).](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-CCND1-809-Flow-Cytometry-NBP2-44586-img0005.jpg)
![Western Blot: RAD18 Antibody [NB100-61063] - Detection of Human RAD18 on HeLa whole cell lysate using NB100-61063. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody NB100-61062.](http://images.novusbio.com/fullsize/RAD18-Antibody-Western-Blot-NB100-61063-img0005.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RAD18 Antibody [NB100-61063] - Detection of Human RAD18 on HeLa whole cell lysate using NB100-61063. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody NB100-61062.")
![Western Blot: RACGAP1 Antibody [NBP2-20049] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Western-Blot-NBP2-20049-img0001.jpg "Western Blot: RACGAP1 Antibody [NBP2-20049] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RACGAP1 Antibody [NBP2-20049] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20049-img0003.jpg)
![Immunohistochemistry-Paraffin: RACGAP1 Antibody [NBP2-20049] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RACGAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20049-img0002.jpg)
![Immunohistochemistry: MgcRacGAP/RACGAP1 Antibody [NBP2-20049] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using racgap1antibody (NBP2-20049) at 1:200 dilution.](http://images.novusbio.com/fullsize/nbp2-20049_rabbit-polyclonal-mgcracgap-racgap1-antibody-91020242021943.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Western-Blot-NBP2-19397-img0002.jpg "Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19397-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] - MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242091420.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242010446.jpg)
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-2622024152247.jpg "Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.")
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-262202415253312.jpg)
![Western Blot: CD44 Antibody (19H12) [NBP3-26680] - Positive Western Blot detected in: A549 whole cell lysate, MCF7 whole cell lysate, HepG2 whole cell lysate. All lanes: CD44 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa Observed band size: 81 kDa](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241136303.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (19H12) [NBP3-26680] - Staining of Hela with CD44 Antibody (19H12) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 515-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241323630.jpg)
![Flow Cytometry: CD44 Antibody (19H12) [NBP3-26680] - Overlay Peak curve showing Hela cells stained with CD44 Antibody (19H12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-282202414312756.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-24504-img0001.jpg "Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.")