| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97592-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97472-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97761-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46881-0.025ml | |||
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Western Blot: AK3L1 Antibody [NBP2-97592] - Lane A: MCF-7 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:27 kDa](http://images.novusbio.com/fullsize/AK3L1-Antibody-Western-Blot-NBP2-97592-img0006.jpg "Western Blot: AK3L1 Antibody [NBP2-97592] - Lane A: MCF-7 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:27 kDa")
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-97592] - Staining of human AK3L1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-97592-img0005.jpg)
![Immunohistochemistry-Paraffin: AK3L1 Antibody [NBP2-97592] - Staining of human AK3L1 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunohistochemistry-Paraffin-NBP2-97592-img0004.jpg)
![Immunoprecipitation: AK3L1 Antibody [NBP2-97592] - Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-AK4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AK4 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size :28 kDa](http://images.novusbio.com/fullsize/AK3L1-Antibody-Immunoprecipitation-NBP2-97592-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AK3L1 Antibody [NBP2-97592] - Immunofluorescence staining of AK3L1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97592 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97592_rabbit-polyclonal-ak3l1-antibody-235202516144341.png)
![Western Blot: MRPL12 Antibody [NBP2-97472] - Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/MRPL12-Antibody-Western-Blot-NBP2-97472-img0006.jpg "Western Blot: MRPL12 Antibody [NBP2-97472] - Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-97472-img0005.jpg)
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human colon with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-97472-img0004.jpg)
![Immunoprecipitation: MRPL12 Antibody [NBP2-97472] - MRPL12 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 ul anti-MRPL12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL12 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunoprecipitation-NBP2-97472-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MRPL12 Antibody [NBP2-97472] - Immunofluorescence staining of MRPL12 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97472 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97472_rabbit-polyclonal-mrpl12-antibody-2352025166477.png)
![Western Blot: FARSLA Antibody [NBP2-97761] - Anti-FARSLA rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa](http://images.novusbio.com/fullsize/FARSLA-Antibody-Western-Blot-NBP2-97761-img0006.jpg "Western Blot: FARSLA Antibody [NBP2-97761] - Anti-FARSLA rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa")
![Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunohistochemistry-Paraffin-NBP2-97761-img0005.jpg)
![Immunohistochemistry-Paraffin: FARSLA Antibody [NBP2-97761] - Immunochemical staining of human FARSLA in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunohistochemistry-Paraffin-NBP2-97761-img0004.jpg)
![Immunoprecipitation: FARSLA Antibody [NBP2-97761] - FARSLA was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-FARSLA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FARSLA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa](http://images.novusbio.com/fullsize/FARSLA-Antibody-Immunoprecipitation-NBP2-97761-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FARSLA Antibody [NBP2-97761] - Immunofluorescence staining of FARSLA in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97761 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97761_rabbit-polyclonal-farsla-antibody-235202516335033.png)
![Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2682024158269.jpg "Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL")
![Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-2982024149267.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-29202410484872.jpg)
![Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32725_mouse-pcna-mab-a6-g11-r-3920248485013.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human liver with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251433153.png)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human pancreas with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-2352025112932.png)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06239 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251633504.png)
![Western Blot: Otx2 Antibody (PSH01-61) [NBP3-32683] - Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. Lane 1: 293T transfected with FLAG-tagged empty control cell lysate Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32683) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-278202415212415.jpg "Western Blot: Otx2 Antibody (PSH01-61) [NBP3-32683] - Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. Lane 1: 293T transfected with FLAG-tagged empty control cell lysate Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32683) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Otx2 Antibody (PSH01-61) [NBP3-32683] - Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32683) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-29820241430410.jpg)
![Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (PSH01-61) [NBP3-32683] - Immunocytochemistry analysis of 293T overexpress with or without Otx2 cells labeling Otx2 with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-29202410484846.jpg)
![Flow Cytometry: Otx2 Antibody (PSH01-61) [NBP3-32683] - Flow cytometric analysis of 293T overexpress with or without Otx2 cells labeling Otx2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32683, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-392024884675.jpg)
![Western Blot: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 monoclonal antibody (M02), clone 1E3. Analysis of RPS23 expression in NIH/3T3.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-Western-Blot-H00006228-M02-img0013.jpg "Western Blot: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 monoclonal antibody (M02), clone 1E3. Analysis of RPS23 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: RPS23 Antibody (1E3) [H00006228-M02] - Analysis of monoclonal antibody to RPS23 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-Immunocytochemistry-Immunofluorescence-H00006228-M02-img0008.jpg)
![Western Blot: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 monoclonal antibody (M02), clone 1E3 Analysis of RPS23 expression in HeLa.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-Western-Blot-H00006228-M02-img0010.jpg)
![Western Blot: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 monoclonal antibody (M02), clone 1E3. Analysis of RPS23 expression in PC-12.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-Western-Blot-H00006228-M02-img0011.jpg)
![Western Blot: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 monoclonal antibody (M02), clone 1E3. Analysis of RPS23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-Western-Blot-H00006228-M02-img0012.jpg)
![ELISA: RPS23 Antibody (1E3) [H00006228-M02] - RPS23 Antibody (1E3) [H00006228-M02] - Detection limit for recombinant GST tagged RPS23 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RPS23-Antibody-1E3-ELISA-H00006228-M02-img0009.jpg)
![Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Western-Blot-NBP2-54545-img0005.jpg "Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).")
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0001.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54545-img0002.jpg)
![Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TOP2A-Antibody-TOP2A-1362-Azide-and-BSA-Free-Protein-Array-NBP2-54545-img0003.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-46881-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-46881] - Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, NBP2-46881, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunofluorescence-NBP2-46881-img0001.jpg)