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![Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Western-Blot-NBP3-15758-img0003.jpg "Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded mouse testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0002.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded rat testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0001.jpg)
![Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Western-Blot-NBP2-67191-img0002.jpg "Western Blot: BMAL1 Antibody (JM17-34) [NBP2-67191] - Analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Rat brain Lane 3: Mouse spleen Lane 4: Hela")
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (JM17-34) [NBP2-67191] - Staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BMAL1-Antibody-JM17-34-Immunocytochemistry-Immunofluorescence-NBP2-67191-img0001.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-49316] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-49316-img0008.jpg "Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-49316] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-49316] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-49316-img0005.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-49316] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-49316-img0006.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-49316] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-49316-img0007.jpg)
![Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CLOCK-Antibody-HL1099-Western-Blot-NBP3-13718-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-910202420115014.jpg)
![Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-91020242041282.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13718_rabbit-clock-mab-hl1099-1610202419405734.jpg)
![Western Blot: CLOCK Antibody (8F7) [NBP1-51610] - Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/CLOCK-Antibody-8F7-Western-Blot-NBP1-51610-img0004.jpg "Western Blot: CLOCK Antibody (8F7) [NBP1-51610] - Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CLOCK Antibody (8F7) [NBP1-51610] - Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CLOCK-Antibody-8F7-Immunocytochemistry-Immunofluorescence-NBP1-51610-img0005.jpg)
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/BMAL1-Antibody-4F5N3-Western-Blot-NBP3-16467-img0001.jpg "Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of extracts of various cell lines, using BMAL1 Rabbit mAb (NBP3-16467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: BMAL1 Antibody (4F5N3) [NBP3-16467] - Western blot analysis of various lysates using BMAL1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16467_rabbit-bmal1-mab-4f5n3-522025125450.jpg)
![Western Blot: BMAL1 Antibody (4F5N3) [BMAL1] - Western blot analysis of various lysates using BMAL1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16467_rabbit-bmal1-mab-4f5n3-52202517273550.jpg)
![Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924283.png "Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C136 (NBP3-20131) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20131, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924232.png)
![Immunohistochemistry: Serotonin N-acetyltransferase Antibody - BSA Free [NBP3-48256] - Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using (Serotonin N-acetyltransferase antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-48256_rabbit-serotonin-n-acetyltransferase-pab-bsa-free-284202511352224.jpg "Immunohistochemistry: Serotonin N-acetyltransferase Antibody - BSA Free [NBP3-48256] - Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using (Serotonin N-acetyltransferase antibody) at dilution of 1:200")
![Western Blot: Serotonin N-acetyltransferase Antibody - BSA Free [NBP3-48256] - Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with (Serotonin N-acetyltransferase Antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-48256_rabbit-serotonin-n-acetyltransferase-pab-bsa-free-284202511425536.jpg)
![Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.](http://images.novusbio.com/fullsize/PRMT5-Antibody-3610-Western-Blot-NBP2-43678-img0003.jpg "Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.")
![Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of Raji whole cell extract A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/PRMT5-Antibody-3610-Immunoprecipitation-NBP2-43678-img0004.jpg)
![Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (NBP2-43678) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43678_mouse-monoclonal-prmt5-antibody-3610-91020242041294.jpg)
![Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PER2-Antibody-2K9Y0-Western-Blot-NBP3-16557-img0003.jpg "Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Chromatin Immunoprecipitation: PER2 Antibody (2K9Y0) [NBP3-16557] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16557_rabbit-per2-mab-2k9y0-5220251255595.jpg)
![Western Blot: BMAL1 Antibody [NBP1-28802] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BMAL1-Antibody-Western-Blot-NBP1-28802-img0001.jpg "Western Blot: BMAL1 Antibody [NBP1-28802] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PER1 Antibody [NBP3-12882] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (NBP3-12882) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PER1-Antibody-Western-Blot-NBP3-12882-img0003.jpg "Western Blot: PER1 Antibody [NBP3-12882] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (NBP3-12882) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: PER1 Antibody [NBP3-12882] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (NBP3-12882) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PER1-Antibody-Western-Blot-NBP3-12882-img0001.jpg)
![Western Blot: PER1 Antibody [NBP3-12882] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (NBP3-12882) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PER1-Antibody-Western-Blot-NBP3-12882-img0002.jpg)