| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56595SS | |||
| Azide and BSA Free | NBP2-33237 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1095SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84896-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41270-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14478-img0003.jpg "Immunocytochemistry/Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining in human testis and skeletal muscle tissues.. Corresponding CHD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0010.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human placenta shows moderate to strong nuclear positivity.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0005.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0006.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0007.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0008.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Western-Blot-NBP2-54613-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - HeLa cells were stained with the antibody against H3K4me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Immunofluorescence-NBP2-54613-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - ChIP assays were performed using human K562 cells, the antibody against H3K4me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-3-Monomethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54613-img0008.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To test the cross reactivity of the antibody against H3K4me1, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Dot-Blot-NBP2-54613-img0003.jpg)
![ELISA: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,300.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-ELISA-NBP2-54613-img0009.jpg)
![Protein Array: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Protein-Array-NBP2-54613-img0010.jpg)
![Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-54620-img0005.jpg "Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - HeLa cells were stained with the antibody against H4K20me3 and with DAPI. Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Immunofluorescence-NBP2-54620-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - ChIP assays were performed using human K562 cells, the antibody against H4K20me3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes EIF4A2 and GAPDH, used as negative controls, and for ZNF510 and the Sat2 satellite repeat region used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys20-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54620-img0001.jpg)
![ELISA: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K20me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,700.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-54620-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To test the cross reactivity of the antibody against H4K20me3. A Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-54620-img0003.jpg)
![Protein Array: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Protein-Array-NBP2-54620-img0004.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-117C826-Western-Blot-NB100-56595-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa")
![Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-western-blot-1132024114213.jpg "Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.")
![Dot Blot analysis of Histone H3 [p Ser10, ac Lys9] Antibody in the peptides listed in 1.0 and 0.5 picomoles of peptide.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-dot-blot-1132024115254..jpg)
![Western Blot: PHC3 Antibody [NBP1-84896] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/PHC3-Antibody-Western-Blot-NBP1-84896-img0005.jpg "Western Blot: PHC3 Antibody [NBP1-84896] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84896_rabbit-polyclonal-phc3-antibody-immunohistochemistry-paraffin-121220238382..jpg)
![Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human testis shows strong nuclear positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp1-84896_rabbit-polyclonal-phc3-antibody-immunohistochemistry-paraffin-121220238398..jpg)
![Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human tonsil shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84896_rabbit-polyclonal-phc3-antibody-immunohistochemistry-paraffin-1212202384016..jpg)
![Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84896_rabbit-polyclonal-phc3-antibody-immunohistochemistry-paraffin-1212202384120..jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NBP2-59180-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NBP2-59180] - ChIP assays were performed using human HeLa cells, the antibody against H3K9ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers speci c for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that acetylation of K9 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59180-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180] - HeLa cells were stained with the antibody against H3K9ac and with DAPI. Cells were mixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immuno uorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunofluorescence-NBP2-59180-img0003.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody [NBP2-59180] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9ac in antigen coated wells. The antigen used was a peptide containing the histone modi cation of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,700.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-ELISA-NBP2-59180-img0005.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NBP2-59187-img0003.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Panel B, C, D and E: staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/uL blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K4me2 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. A: cells were immunofluorescently labeled with the H3K4me2 antibody (left) diluted 1:5,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - ChIP was performed with the antibody against H3K4me2 on sheared chromatin from 500,000 HeLaS3 cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration of the antibody of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0006.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K4me2 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:48,200.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-ELISA-NBP2-59187-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me2 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59187-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~18 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Western-Blot-NBP2-59198-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~18 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - HeLa cells were stained with the Diagenode antibody against H3K9me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9me3 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59198-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using the](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59198-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me3 with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Dot-Blot-NBP2-59198-img0007.jpg)
![ELISA: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-ELISA-NBP2-59198-img0009.jpg)
![Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NBP2-41270-img0001.jpg "Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NBP2-41270-img0005.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunofluorescence-NBP2-41270-img0004.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-2711202420141992.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-27112024201313.jpg)
![Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Western-Blot-NBP3-14696-img0004.jpg "Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696] - Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Immunocytochemistry-Immunofluorescence-NBP3-14696-img0005.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696] - Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Immunohistochemistry-Paraffin-NBP3-14696-img0002.jpg)
![Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/Brg1-Antibody-BLR106H-Western-Blot-NBP3-14696-img0003.jpg)
![Immunoprecipitation: Brg1 Antibody [NBP3-14696] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRG1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. BRG1 was alsoimmunoprecipitated by rabbit anti-BRG1 antibody. For blotting immunoprecipitated BRG1, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NBP3-14696-img0001.jpg)
![Western Blot: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Western blot analysis of extracts of various cell lines, using BAF180/PB1 Rabbit mAb (NBP3-15258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Western-Blot-NBP3-15258-img0004.jpg "Western Blot: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Western blot analysis of extracts of various cell lines, using BAF180/PB1 Rabbit mAb (NBP3-15258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded mouse kidney using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0003.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded rat ovary using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0001.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded human appendix using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0002.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human colon using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-72202512443311.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-72202512435353.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human spleen using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-7220251245372.jpg)