| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1164AF405 | |||
| Alexa Fluor 488 | NB21-1164AF488 | |||
| Alexa Fluor 647 | NB21-1164AF647 | |||
| Alexa Fluor 700 | NB21-1164AF700 | |||
| Biotin | NB21-1164B | |||
| DyLight 405 | NB21-1164V | |||
| DyLight 488 | NB21-1164G | |||
| DyLight 550 | NB21-1164R | |||
| DyLight 650 | NB21-1164C | |||
| FITC | NB21-1164F | |||
| HRP | NB21-1164H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1164SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1273V | |||
| FITC | NB21-1273F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1273SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33834-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24576-25ul | |||
| Azide and BSA Free | NBP3-43830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43955 | |||
| BSA Free | NBP2-30673-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25995 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02966-20ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1254AF405 | |||
| Alexa Fluor 488 | NB21-1254AF488 | |||
| Alexa Fluor 647 | NB21-1254AF647 | |||
| Alexa Fluor 700 | NB21-1254AF700 | |||
| Biotin | NB21-1254B | |||
| DyLight 405 | NB21-1254V | |||
| DyLight 488 | NB21-1254G | |||
| DyLight 550 | NB21-1254R | |||
| DyLight 650 | NB21-1254C | |||
| FITC | NB21-1254F | |||
| HRP | NB21-1254H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1254SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1001AF405 | |||
| Alexa Fluor 488 | NB21-1001AF488 | |||
| Alexa Fluor 647 | NB21-1001AF647 | |||
| Alexa Fluor 700 | NB21-1001AF700 | |||
| Biotin | NB21-1001B | |||
| DyLight 405 | NB21-1001V | |||
| DyLight 488 | NB21-1001G | |||
| DyLight 550 | NB21-1001R | |||
| DyLight 650 | NB21-1001C | |||
| FITC | NB21-1001F | |||
| HRP | NB21-1001H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1001SS | |||
![Dot Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Dot blot analysis of Histone H3 K23ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys23]-Antibody-Dot-Blot-NB21-1164-img0001.jpg "Dot Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Dot blot analysis of Histone H3 K23ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Western blot analysis of Histone H3 K23ac in 1. NIH 3T3 histone prep lysate and 2. HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys23]-Antibody-Western-Blot-NB21-1164-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 in (1) HeLa histone prep and (2) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys37]-Antibody-Western-Blot-NB21-1273-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 in (1) HeLa histone prep and (2) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys37]-Antibody-Dot-Blot-NB21-1273-img0005.jpg)
![Western Blot: SUZ12 Antibody [NBP2-33834] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/SUZ12-Antibody-Western-Blot-NBP2-33834-img0004.jpg "Western Blot: SUZ12 Antibody [NBP2-33834] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)")
![Immunocytochemistry/Immunofluorescence: SUZ12 Antibody [NBP2-33834] - Staining of human cell line HEK 293 shows localization to nucleoli & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SUZ12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33834-img0006.jpg)
![Immunohistochemistry-Paraffin: SUZ12 Antibody [NBP2-33834] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SUZ12-Antibody-Immunohistochemistry-Paraffin-NBP2-33834-img0010.jpg)
![Immunohistochemistry-Paraffin: SUZ12 Antibody [NBP2-33834] - Staining of human tonsil shows weak nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/SUZ12-Antibody-Immunohistochemistry-Paraffin-NBP2-33834-img0008.jpg)
![Immunohistochemistry-Paraffin: SUZ12 Antibody [NBP2-33834] - Staining of human small intestine shows weak nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SUZ12-Antibody-Immunohistochemistry-Paraffin-NBP2-33834-img0009.jpg)
![Immunohistochemistry-Paraffin: SUZ12 Antibody [NBP2-33834] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/SUZ12-Antibody-Immunohistochemistry-Paraffin-NBP2-33834-img0011.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Western-Blot-NBP2-30673-img0013.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0016.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0017.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0018.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0020.jpg)
![Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Western-Blot-NBP2-61538-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Immunohistochemistry staining of HepG2 cells using NBP2-61538.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Immunohistochemistry-NBP2-61538-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP2-61538] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM166 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61538_rabbit-monoclonal-histone-h3-ac-lys18-antibody-rm166-immunocytochemistry-immunofluorescence-18920252348533.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP2-61538] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using Clone RM166 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61538_rabbit-monoclonal-histone-h3-ac-lys18-antibody-rm166-chromatin-immunoprecipitation-1892025233959.png)
![ELISA: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and biotinylated anti-H3K18ac NBP2-61538 at 2ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-ELISA-NBP2-61538-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32431) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-26820241525101.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32431) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-278202416331219.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (positive) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (negative) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Proteins loading: 100ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 15 seconds; ECL.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-28820241459255.jpg)
![Immunohistochemistry: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32431) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-29820241492625.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Immunocytochemistry analysis of HeLa cells labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-2920241192379.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Western-Blot-NBP2-59186-img0002.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4me3 antibody (left) diluted 1:100 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59186-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3 modifications and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me3 modification. At higher concentrations some H3K4me2 is also precipitated.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - ChIP assays were performed using human HeLa cells, the Ab against H3K4me3 and PCR primers for qPCR. ChIP was performed using sheared chromatin from 1 million cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me3 with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59186-img0003.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NBP2-59200-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Mouse NIH3T3 cells were stained with the antibody against H3K9me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunofluorescence-NBP2-59200-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me2 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59200-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9me2 , crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-ELISA-NBP2-59200-img0002.jpg)
![Western Blot: CHD1 Antibody [NBP3-02966] - Analysis of extracts of Jurkat cells, using CHD1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NBP3-02966-img0006.jpg "Western Blot: CHD1 Antibody [NBP3-02966] - Analysis of extracts of Jurkat cells, using CHD1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Rat brain using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0003.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Mouse brain using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Human liver damage using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NBP3-02966] - Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (NBP3-02966) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockout-Validated-NBP3-02966-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of H3 K36ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Dot-Blot-NB21-1254-img0001.jpg "Dot Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of H3 K36ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of Histone H3 K36ac in C. elegans embryo lysate. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Western-Blot-NB21-1254-img0002.jpg)
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~14 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Arg2]-Antibody-Western-Blot-NB21-1001-img0007.jpg "Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~14 kDa.")
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Arg2]-Antibody-Western-Blot-NB21-1001-img0006.jpg)