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![Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K79me3, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NBP2-59205-img0007.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K79me3, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - ChIP was performed with the antibody against H3K79me3 on sheared chromatin from 1 million HeLaS3 cells using a ChIP-seq kit. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59205-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K79me3 with peptides containing other histone modifications and the unmodified H3K79. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:100,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Dot-Blot-NBP2-59205-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against H3K79me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-ELISA-NBP2-59205-img0006.jpg)
![Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/WDR5-Antibody-Western-Blot-NBP1-30070-img0003.jpg "Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- WDR5 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/WDR5-Antibody-Immunohistochemistry-NBP1-30070-img0002.jpg)
![Western Blot: WDR5 Antibody [NBP1-30070] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. WDR5 was successfully IPed by NBP1-30063.](http://images.novusbio.com/fullsize/WDR5-Antibody-Western-Blot-NBP1-30070-img0001.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49955-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49955] - Detection of Human Sequestosome-1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize other epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49955] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49955-img0003.jpg)
![Western Blot: WDR5 Antibody [NBP3-18669] - Figure 3. Western blot analysis using the antibody directed against WDR5 Nclear extracts from HeLa cells (20 ug) were analysed by Western blot using the antibody against WDR5 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/WDR5-Antibody-Western-Blot-NBP3-18669-img0001.jpg "Western Blot: WDR5 Antibody [NBP3-18669] - Figure 3. Western blot analysis using the antibody directed against WDR5 Nclear extracts from HeLa cells (20 ug) were analysed by Western blot using the antibody against WDR5 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: WDR5 Antibody [NBP3-18669] - Figure 4. Immunofluorescence using the antibody directed against WDR HeLa cells were stained with the antibody against WDR5 and with DAPI. Cells were fixed with 4% formaldehyde for 10 and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/WDR5 Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18669-img0003.jpg)
![Chromatin Immunoprecipitation (ChIP): WDR5 Antibody [NBP3-18669] - Figure 1. ChIP results obtained with antibody directed against WDR5. ChIP assays performed using HeLa cells, antibody against WDR5 and optimized PCR primer sets for qPCR. ChIP performed with the](http://images.novusbio.com/fullsize/WDR5-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP3-18669-img0004.jpg)
![Chromatin Immunoprecipitation Sequencing: WDR5 Antibody [NBP3-18669] - Figure 2. ChIP-seq results obtained with the antibody directed against WDR5 ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 ug of the antibody against WDR5 as described above. The IPd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturers instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the RPL41 and RPS4X positive control genes (fig 2C and D).](http://images.novusbio.com/fullsize/WDR5-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP3-18669-img0002.jpg)
![Western Blot: AEBP2 Antibody [NBP2-30712] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Western-Blot-NBP2-30712-img0005.jpg "Western Blot: AEBP2 Antibody [NBP2-30712] - Analysis in human cell line BEWO.")
![Immunocytochemistry/Immunofluorescence: AEBP2 Antibody [NBP2-30712] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30712-img0004.jpg)
![Immunohistochemistry: AEBP2 Antibody [NBP2-30712] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AEBP2-Antibody-Immunohistochemistry-NBP2-30712-img0003.jpg)
![Western Blot: AEBP2 Antibody [NBP2-30712] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/AEBP2-Antibody-Western-Blot-NBP2-30712-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMARCC2-BAF170-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90017-img0007.jpg "Immunocytochemistry/Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Western-Blot-NBP2-59217-img0001.jpg "Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - ChIP assays were performed using human HeLa cells, treated with TSA, the antibody against H3K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NBP2-59217-img0003.jpg)
![ELISA: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K18ac . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-ELISA-NBP2-59217-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - HeLa cells were stained with the antibody against H3K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18-Dimethyl-Arg17]-Antibody-Immunofluorescence-NBP2-59217-img0004.jpg)
![Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-278202415295572.jpg "Western Blot: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Western blot analysis of p62/SQSTM1 on different lysates with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/500 dilution. Lane 1: PC-12 cell lysate (10 ug/Lane) Lane 2: RAW264.7 cell lysate (10 ug/Lane) Lane 3: PANC-1 cell lysate (10 ug/Lane) Lane 4: Mouse liver tissue lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32702) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32702) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-298202414161843.jpg)
![Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (PS00-61) [NBP3-32702] - Immunocytochemistry analysis of A549 cells labeling p62/SQSTM1 with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p62/SQSTM1 antibody (NBP3-32702) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32702_rabbit-p62-sqstm1-mab-ps00-61-29202410484897.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Western-Blot-NBP2-59294-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Immunofluorescence-NBP2-59294-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K9me3 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 3 and 9 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Chromatin-Immunoprecipitation-NBP2-59294-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Cross reactivity of the antibody directed against H3K9me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K9me2. The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-ELISA-NBP2-59294-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Western-Blot-NBP2-59295-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Immunofluorescence-NBP2-59295-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer sets for qPCR. ChIP was performed with the AutoHistone ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Chromatin-Immunoprecipitation-NBP2-59295-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Cross reactivity of the antibody directed against H3K27me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K27me3. The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-ELISA-NBP2-59295-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - ChIP assays were performed using human HeLa cells treated with colcemid, the antibody against H3K27me3S28p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27-p-Ser28]-Antibody-Chromatin-Immunoprecipitation-NBP2-59212-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Western blot was performed on histone extracts (15 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 2) using the antibody against H3K27me3S28p diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me3S28p. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-ELISA-NBP2-59212-img0001.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3S28p with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27S28 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:2,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Dot-Blot-NBP2-59212-img0004.jpg)
![Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924296.png "Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C223 (NBP3-20133) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924242.png)
![Immunocytochemistry/Immunofluorescence: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C223 (NBP3-20133) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20133, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023964611.png)