| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89218-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33102 | |||
| IHC-Prediluted | NBP2-44724 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89756-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47858-0.1mg | |||
| IHC-Prediluted | NBP2-48223 | |||
![Western Blot: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Cate](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Western-Blot-NBP2-70509-img0002.jpg "Western Blot: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Cate")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY beta Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70509-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0011.jpg)
![Flow Cytometry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-beta Catenin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70509-img0004.jpg)
![Western Blot: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Western-Blot-NBP2-70509-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0006.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0007.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0008.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0010.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70509-img0012.jpg)
![Flow Cytometry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI12H7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70509-img0003.jpg)
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0006.jpg "Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0009.jpg)
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Immunocytochemistry-Immunofluorescence-NB600-1409-img0008.jpg "Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.")
![Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Flow-Cytometry-NB600-1409-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human lymphoma with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD45 Antibody (101) [NBP2-89218] - Staining of human LCA in human spleen with rabbit monoclonal CD45 antibody (101) [NBP2-89218] at 1:200 dilution.](http://images.novusbio.com/fullsize/CD45-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89218-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0010.jpg "Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0007.jpg)
![Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Flow-Cytometry-NBP2-67390-img0001.jpg)
![Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Western-Blot-NBP2-67390-img0008.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0004.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0005.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCNA-Antibody-SY12-07-Immunohistochemistry-Paraffin-NBP2-67390-img0006.jpg)
![Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Western-Blot-NBP2-67326-img0008.jpg "Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0003.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BS](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0009.jpg)
![Flow Cytometry: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Flow-Cytometry-NBP2-67326-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0006.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0007.jpg)
![Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Western-Blot-NBP2-67078-img0008.jpg "Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0004.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0007.jpg)
![Flow Cytometry: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Flow-Cytometry-NBP2-67078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0005.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in RH-35 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling alpha-smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution and ALDH2 (green) at 1/100 dilution overnight at 4 , washed with PBS. iFluor(TM) 488 conjugate-Goat anti-Mouse IgG and iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0012.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of Jurkat cells with alpha-Smooth Muscle Actin Antibody (SY02-64) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Flow-Cytometry-NBP2-67440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A431 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in A549 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Staining alpha-Smooth Muscle Actin in HepG2 cells with alpha-Smooth Muscle Actin Antibody (SY02-64) (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunocytochemistry-Immunofluorescence-NBP2-67440-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human gastric carcinoma tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human liver tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of paraffin-embedded human tonsil tissue using alpha-Smooth Muscle Actin Antibody (SY02-64). Counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Immunofluorescence analysis of paraffin-embedded human Kidney tissue labeling alpha smooth muscle Actin (washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 , washed with PBS. iFluor(TM) 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SY02-64-Immunohistochemistry-Paraffin-NBP2-67440-img0011.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (SY02-64) [NBP2-67440] - Analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.Lane 1: Hela-si NT cell lysate (10 ug/Lane)Lane 2: Hela-si alpha smooth muscle Actin cell lysate (10 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 5 seconds;4-20% SDS-PAGE gel. was shown to specifically react with alpha smooth muscle Actin in Hela-si NT cells. Weakened band was observed when Hela-si alpha smooth muscle Actin sample was tested. Hela-si NT and Hela-si alpha smooth muscle Actin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67440_rabbit-monoclonal-alpha-smooth-muscle-actin-antibody-sy02-64-western-blot-2372024113558..jpg)
![Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Western-Blot-NBP2-29629-img0007.jpg "Western Blot: NF-H Antibody (NF421) [NBP2-29629] - Western Blot Analysis of Human Brain Tissue Lysate using NF-H antibody (NF421).")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (NF421) [NBP2-29629] - Immunofluorescence staining of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunocytochemistry-Immunofluorescence-NBP2-29629-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (NF421).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0006.jpg)
![Flow Cytometry: NF-H Antibody (NF421) [NBP2-29629] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Flow-Cytometry-NBP2-29629-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-91).](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with neurofilament monoclonal Antibody (RT-97)](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NF421) [NBP2-29629] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament NF421 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/NF-H-Antibody-NF421-Immunohistochemistry-Paraffin-NBP2-29629-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89756-img0012.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP1-89756] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Western Blot: GM130/GOLGA2 Antibody [NBP1-89756] - Analysis using Anti-GOLGA2 antibody NBP1-89756 (A) shows similar pattern to independent antibody NBP1-89758 (B).](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-NBP1-89756-img0014.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Immunohistochemistry-Paraffin-NBP2-44607-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-1772024100320.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-177202495943.jpg)
![Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody NB100-604 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Western-Blot-NB100-604-img0013.jpg "Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody NB100-604 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS (NB100-604). Detection: DAB](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Immunohistochemistry-NB100-604-img0011.jpg)
![Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS on HeLa whole cell lysates using NB100-604. IP of PNUTS was successfully performed with NB100-603, NB100-605, and another PNUTS polyclonal. IPed PNUTS was blotted using NB100-605.](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Western-Blot-NB100-604-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody NB100-604 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Western-Blot-NB100-604-img0010.jpg)
![Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Immunohistochemistry-NB100-604-img0007.jpg)
![Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Immunohistochemistry-NB100-604-img0008.jpg)
![Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Examples of PNUTS staining: (a) comparison of PNUTS/PPP1R10 staining in (1) normal and (2) cancerous prostate glands of the same TMA spot. Cancer spots with (b) lack of staining and (c) weak, (d) moderate, and (e) strong staining. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Immunohistochemistry-NB100-604-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody NB100-604 (lot NB100-604-3) used for IP at 3 ug per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (lot NB100-604-2) and rabbit anti-PNUTS antibody NB100-605. For blotting immunoprecipitated PNUTS, NB100-604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PNUTS-PPP1R10-Antibody-Immunoprecipitation-NB100-604-img0012.jpg)
![Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Examples of PNUTS staining: (a) comparison of PNUTS staining in (1) normal & (2) cancerous prostate glands of the same TMA spot. Cancer spots with (b) lack of staining & (c) weak, (d) moderate, & (e) strong staining. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32377272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-604_rabbit-polyclonal-pnuts-ppp1r10-antibody-310202415291995.jpg)