| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97931SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84806-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03701-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49538-25ul | |||
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0008.jpg "Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ERF Antibody [NBP2-16366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NBP2-16366-img0006.jpg)
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - U373 xenograft, using ERF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-Paraffin-NBP2-16366-img0002.jpg)
![Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIF1-Antibody-Immunohistochemistry-NBP1-97931-img0001.jpg "Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.")
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0008.jpg "Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of monoclonal antibody to MAPK6 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Immunocytochemistry-Immunofluorescence-H00005597-M01-img0005.jpg)
![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0006.jpg)
![Sandwich ELISA: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Sandwich-ELISA-H00005597-M01-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84806-img0006.jpg "Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP1-84806] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP1-84806-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-84806_rabbit-polyclonal-erk3-mapk6-antibody-104202515321338.jpg)
![TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41217_mouse-monoclonal-trf-1-antibody-4e4-immunocytochemistry-immunofluorescence-261120251838405.jpg "TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-278202415212460.jpg "Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-2920241192388.jpg)
![Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-392024884681.jpg)
![Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-NB100-88120-img0004.jpg "Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ERF Antibody [NB100-88120] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88120 used for IP at 3 mcg/mg lysate. ERF was also immunoprecipitated by NB100-88119, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ERF-Antibody-Western-Blot-NB100-88120-img0003.jpg)
![Western Blot: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of extracts of various cell lines, using ERK3/MAPK6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Western-Blot-NBP3-03701-img0003.jpg "Western Blot: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of extracts of various cell lines, using ERK3/MAPK6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of L929 cells using ERK3/MAPK6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03701-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-49538] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49538-img0002.jpg "Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-49538] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: ERF Antibody [NBP2-49538] - Staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ERF-Antibody-Immunohistochemistry-NBP2-49538-img0001.jpg)