| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16148-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92161-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38191-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27148-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32614-25ul | |||
![Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-Antibody-Immunohistochemistry-Paraffin-NB100-204-img0008.jpg "Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-Antibody-Immunohistochemistry-Paraffin-NB100-204-img0007.jpg)
![Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SMC1-Antibody-Simple-Western-NB100-204-img0006.jpg)
![SMC1 Antibody [NB100-204] - Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC1 antibody NB100-204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Previous](http://images.novusbio.com/fullsize/SMC1-Antibody-NB100-204-img0009.jpg)
![SMC1 Antibody [NB100-204] - Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC1 antibody NB100-204 used for IP at 6 ug per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 recombinant monoclonal antibody NB2-76412. For blotting immunoprecipitated SMC1, NB100-204 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.](http://images.novusbio.com/fullsize/SMC1-Antibody-NB100-204-img0010.jpg)
![Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Western-Blot-NB100-206-img0006.jpg "Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.")
![Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-206-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0007.jpg)
![SMC1 [p Ser966] Antibody [NB100-206] - Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody NB100-206 used for IP at 3 ul per reaction. Phospho SMC1 (S966) was also immunoprecipitated by rabbit anti-SMC1 antibody NB100-204. For blotting immunoprecipitated Phospho SMC1 NB100-206 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody NB100-204 was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-NB100-206-img0009.jpg)
![Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-POLD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-POLD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90925-img0010.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0018.jpg)
![Western Blot: DNA polymerase delta p50 Antibody [NBP1-90925] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Western-Blot-NBP1-90925-img0011.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0013.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0014.jpg)
![Immunohistochemistry-Paraffin: DNA polymerase delta p50 Antibody [NBP1-90925] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNA-polymerase-delta-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-90925-img0016.jpg)
![Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NB100-58788-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NB100-58788-img0003.jpg)
![Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Western-Blot-NBP3-16148-img0002.jpg "Western Blot: JNK1/2/3 Antibody [NBP3-16148] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (NBP3-16148) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: JNK1/2/3 Antibody [NBP3-16148] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug JNK1/2/3 antibody (NBP3-16148). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (NBP3-16148) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-Immunoprecipitation-NBP3-16148-img0003.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-5220251255553.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-522025125443.jpg)
![Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511392252.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16148_rabbit-jnk1-2-3-pab-52202511361224.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg "Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.")
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0005.jpg "Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunocytochemistry/Immunofluorescence: HBO1 Antibody [NBP1-92161] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92161-img0007.jpg)
![Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161] - Staining of human stomach shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunohistochemistry-Paraffin-NBP1-92161-img0002.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0004.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92161_rabbit-polyclonal-hbo1-antibody-295202516464821.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411421032.jpg "Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411421073.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411343384.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-101220241136061.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-101220241136069.jpg)
![Western Blot: HBO1 Antibody [NBP3-38191] - Western blot analysis of various lysates using HBO1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411364661.jpg)
![Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-26820241582610.jpg "Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL")
![Immunohistochemistry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32740) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-298202414262615.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-292024111484.jpg)
![Flow Cytometry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Flow cytometric analysis of HeLa cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-29202416372132.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/50,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Lane 4: Mouse liver tissue lysate (15 µg/Lane) Lane 5: Mouse kidney tissue lysate (15 µg/Lane) Lane 6: Rat liver tissue lysate (15 µg/Lane) Lane 7: Rat kidney tissue lysate (15 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-3920248453533.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0002.jpg "Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/JWA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12158-img0003.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Small Intestine with antibody at 3.75 g/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0006.jpg)
![Flow Cytometry: JWA Antibody [NBP3-12158] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/JWA-Antibody-Flow-Cytometry-NBP3-12158-img0004.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0001.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Tonsil with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0005.jpg)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491704.jpg%3Fv=1666704274 "Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (1D9H9) [NBP3-27148] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916212.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded cervical cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202492021.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916234.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024917033.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded colon cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491629.jpg%3Fv=1666704274)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Analysis in human placenta and liver tissues using NBP2-32614 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Analysis in human placenta and liver tissues using NBP2-32614 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human cerebellum shows no positivity in purkinje cells as expected.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0001.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human testis shows moderate to strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXO4 Antibody [NBP2-32614] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FOXO4-Antibody-Immunohistochemistry-Paraffin-NBP2-32614-img0004.jpg)
