| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52448-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52578-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37312-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12579-50ul | |||
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0004.jpg "Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)")
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0003.jpg)
![Flow Cytometry: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Flow-Cytometry-NBP2-52448-img0001.jpg)
![Western Blot: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Western-Blot-NBP2-52448-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: G-CSF Antibody (8G5F7) [NBP2-52448] - Analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-Immunofluorescence-NBP2-52448-img0002.jpg)
![ELISA: G-CSF Antibody (8G5F7) [NBP2-52448] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/G-CSF-Antibody-8G5F7-ELISA-NBP2-52448-img0006.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Western-Blot-NBP2-52578-img0002.jpg "Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)")
![Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded colon cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Immunohistochemistry-Paraffin-NBP2-52578-img0006.jpg)
![Flow Cytometry: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of Hela cells using TUBE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Flow-Cytometry-NBP2-52578-img0001.jpg)
![Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - 2: Western blot analysis using TUBE1 mAb against HEK293 (1) and TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Western-Blot-NBP2-52578-img0003.jpg)
![Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded liver cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-Immunohistochemistry-Paraffin-NBP2-52578-img0007.jpg)
![ELISA: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Epsilon-1-Tubulin-Antibody-5F3B7-ELISA-NBP2-52578-img0004.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0004.jpg "Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.")
![Immunohistochemistry: p38 Antibody (10B11C8) [NBP2-37312] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Immunohistochemistry-NBP2-37312-img0005.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0002.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0003.jpg)
![ELISA: p38 Antibody (10B11C8) [NBP2-37312] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-ELISA-NBP2-37312-img0001.jpg)
![Western Blot: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2.](http://images.novusbio.com/fullsize/Beta-1-3-N-Acetylglucosaminyltransferase-2-B3GNT2-Antibody-OTI3A9-Azide-and-BSA-Free-Western-Blot-NBP2-71624-img0001.jpg "Western Blot: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2.")
![Immunocytochemistry/Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2.](http://images.novusbio.com/fullsize/Beta-1-3-N-Acetylglucosaminyltransferase-2-B3GNT2-Antibody-OTI3A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71624-img0004.jpg)
![Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Beta-1-3-N-Acetylglucosaminyltransferase-2-B3GNT2-Antibody-OTI3A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71624-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Beta-1-3-N-Acetylglucosaminyltransferase-2-B3GNT2-Antibody-OTI3A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71624-img0005.jpg)
![Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) - Azide and BSA Free [NBP2-71624] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Beta-1-3-N-Acetylglucosaminyltransferase-2-B3GNT2-Antibody-OTI3A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71624-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)
![Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-70383-img0001.jpg "Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.")
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0006.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0002.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0003.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0004.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0005.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0007.jpg)
![Western Blot: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI-1.](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Western-Blot-NBP2-70827-img0001.jpg "Western Blot: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI-1.")
![Immunohistochemistry: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of Human Kidney tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70827-img0005.jpg)
![Immunohistochemistry: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70827-img0002.jpg)
![Immunohistochemistry: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70827-img0003.jpg)
![Immunohistochemistry: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70827-img0004.jpg)
![Immunohistochemistry: GFI-1 Antibody (OTI6E8) - Azide and BSA Free [NBP2-70827] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GFI-1-Antibody-OTI6E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70827-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)
![Western Blot: PTRH2 Antibody [NBP3-12579] - Lane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PTRH2-Antibody-Western-Blot-NBP3-12579-img0001.jpg "Western Blot: PTRH2 Antibody [NBP3-12579] - Lane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human liver with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunohistochemistry-Paraffin-NBP3-12579-img0003.jpg)
![Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunohistochemistry-Paraffin-NBP3-12579-img0002.jpg)
![Immunoprecipitation: PTRH2 Antibody [NBP3-12579] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PTRH2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PTRH2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size :19 kDa](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunoprecipitation-NBP3-12579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PTRH2 Antibody [NBP3-12579] - Staining of PTRH2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12579 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12579_rabbit-polyclonal-ptrh2-antibody-235202514371335.png)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)