| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48090 | |||
| Azide and BSA Free | NBP2-86683 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47739-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26313-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC909) [NBP2-45146] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC909).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC909-Immunohistochemistry-Paraffin-NBP2-45146-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC909) [NBP2-45146] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC909).")
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0005.jpg "Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast tissue. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used at at dilution of 1:250 and incubated for 18 hours at room temperature. Detection: DAB")
![Immunohistochemistry-Paraffin: PCNA [p Tyr211] Antibody [NB100-59861] - Samples: FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-PCNA (Y211). Antibody: Affinity purified rabbit anti-Phospho-PCNA (Y211) used a dilution of 1:250 and incubated for 18 hours at room temeprature. Detection: DAB](http://images.novusbio.com/fullsize/PCNA-[p-Tyr211]-Antibody-Immunohistochemistry-NB100-59861-img0004.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275 + MYC909) [NBP2-45147] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC275 + MYC909).](http://images.novusbio.com/fullsize/c-Myc-Antibody-MYC275-+-MYC909-Immunohistochemistry-Paraffin-NBP2-45147-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (MYC275 + MYC909) [NBP2-45147] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (MYC275 + MYC909).")
![Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30481-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.")
![Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-262202414471594.jpg "Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-262202415475610.jpg)
![Western Blot: SMC1 Antibody (8C11) [NBP3-26313] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate. All lanes: SMC1 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 144 kDa Observed band size: 144 kDa](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-282202411442617.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Western Blot: NEK4 Antibody [NBP1-42687] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NEK4-Antibody-Western-Blot-NBP1-42687-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NEK4 Antibody [NBP1-42687] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NEK4 Antibody [NBP1-42687] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-NBP1-42687-img0002.jpg)
![Western Blot: AZ1 Antibody [NB100-68227] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68227. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 antibodies NB100-68225 and NB100-68226.](http://images.novusbio.com/fullsize/AZ1-Antibody-Western-Blot-NB100-68227-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AZ1 Antibody [NB100-68227] - Detection of Human CEP131/AZ1 on HeLa whole cell lysate using NB100-68227. CEP131/AZ1 was also immunoprecipitated by rabbit anti-CEP131/AZ1 antibodies NB100-68225 and NB100-68226.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: AZ1 Antibody [NB100-68227] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/AZ1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-68227-img0003.jpg)
![Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-NBP1-42686-img0003.jpg "Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: NEK4 Antibody [NBP1-42686] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42687, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunoprecipitation-NBP1-42686-img0002.jpg)
![Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0052.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235]](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0001.jpg)
![Western Blot: RFC5 Antibody [NB100-235] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0002.jpg)