| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77330-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94045-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61719-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26313-50ul | |||
![Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-77330-img0005.jpg "Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Borealin Antibody - BSA Free [NBP1-77330] - Immunofluorescence of Borealin in Rat Kidney cells with Borealin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77330_rabbit-polyclonal-borealin-antibody-2711202419353815.jpg)
![Western Blot: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of extracts of various cell lines, using SCP2/SYCP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/SCP2-SYCP2-Antibody-Western-Blot-NBP2-94045-img0002.jpg "Western Blot: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of extracts of various cell lines, using SCP2/SYCP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: SCP2/SYCP2 Antibody [NBP2-94045] - Analysis of C6 cells using SCP2/SYCP2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SCP2-SYCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94045-img0001.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0003.jpg "Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)")
![Flow Cytometry: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Flow-Cytometry-NBP2-61719-img0002.jpg)
![Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-Western-Blot-NBP2-61719-img0004.jpg)
![ELISA: Bub1 Antibody (1A11C7) [NBP2-61719] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Bub1-Antibody-1A11C7-ELISA-NBP2-61719-img0001.jpg)
![Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0010.jpg "Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.")
![Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SCC112-Antibody-Western-Blot-NB100-426-img0009.jpg)
![Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NB100-93320-img0006.jpg "Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Immunoprecipitation-NB100-93320-img0007.jpg)
![Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0002.jpg "Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.")
![Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://images.novusbio.com/fullsize/NIPBL-Antibody-Western-Blot-NBP1-77974-img0001.jpg)
![Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0052.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235]](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0001.jpg)
![Western Blot: RFC5 Antibody [NB100-235] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0002.jpg)
![Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0089.jpg "Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0088.jpg)
![Western Blot: SMC5 Antibody [NBP2-20419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (NBP2-20419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20419_rabbit-polyclonal-smc5-antibody-910202420122016.jpg "Western Blot: SMC5 Antibody [NBP2-20419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (NBP2-20419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (NBP2-20419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-20419_rabbit-polyclonal-smc5-antibody-western-blot-261120251850421.jpg)
![Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-262202414471594.jpg "Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-262202415475610.jpg)
![Western Blot: SMC1 Antibody (8C11) [NBP3-26313] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate. All lanes: SMC1 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 144 kDa Observed band size: 144 kDa](http://images.novusbio.com/fullsize/nbp3-26313_rabbit-smc1-mab-8c11-282202411442617.jpg)