| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03993SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47778-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08795 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90329-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14276-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30589-25ul | |||
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Western-Blot-NBP1-97587-img0006.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Flow-Cytometry-NBP1-97587-img0004.jpg)
![Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)](http://images.novusbio.com/fullsize/STIM1-Antibody-CDN3H4-Western-Blot-NB110-55300-img0003.jpg "Western Blot: STIM1 Antibody (CDN3H4) [NB110-55300] - (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)")
![Immunocytochemistry/Immunofluorescence: STIM1 Antibody (CDN3H4) [NB110-55300] - Staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor(R) 598; red). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/STIM1-Antibody-CDN3H4-Immunocytochemistry-Immunofluorescence-NB110-55300-img0004.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg "Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Knockout-Validated-NBP2-44514-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-44514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Western-Blot-NBP2-44514-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Confocal Immunofluorescence image of Raji cells using B7-2/CD86 Antibody (C86/1146) followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunocytochemistry-Immunofluorescence-NBP2-44514-img0007.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (C86/1146) [NBP2-44514] - Human Melanoma stained with CD86 Monoclonal Antibody (C86/1146).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-C86-1146-Immunohistochemistry-Paraffin-NBP2-44514-img0002.jpg)
![Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - AKAP8 monoclonal antibody (M01), clone 3D4 Analysis of AKAP8 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Western-Blot-H00010270-M01-img0012.jpg "Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - AKAP8 monoclonal antibody (M01), clone 3D4 Analysis of AKAP8 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Immunocytochemistry-Immunofluorescence-H00010270-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Immunohistochemistry-Paraffin-H00010270-M01-img0009.jpg)
![Western Blot: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4.Lane 1: AKAP8 transfected lysate(76.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Western-Blot-H00010270-M01-img0013.jpg)
![Immunoprecipitation: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Analysis of AKAP8 transfected lysate using anti-AKAP8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKAP8 monoclonal antibody.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Immunoprecipitation-H00010270-M01-img0010.jpg)
![Sandwich ELISA: AKAP95/AKAP8 Antibody (3D4) [H00010270-M01] - Detection limit for recombinant GST tagged AKAP8 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-3D4-Sandwich-ELISA-H00010270-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (rC81/3442) [NBP3-07474] - Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (r1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-rC81-3442-Immunohistochemistry-Paraffin-NBP3-07474-img0002.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (rC81/3442) [NBP3-07474] - Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (r1.3.3.22).")
![Flow Cytometry: CD81 Antibody (rC81/3442) [NBP3-07474] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 antibody (rC81/3442) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-rC81-3442-Flow-Cytometry-NBP3-07474-img0003.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (rC81/3442) [NBP3-07474] - Formalin-fixed, paraffin-embedded human tonsil stained with CD81 Recombinant Mouse Monoclonal Antibody (r1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-rC81-3442-Immunohistochemistry-Paraffin-NBP3-07474-img0001.jpg)
![Flow Cytometry: CD81 Antibody (rC81/3442) [NBP3-07474] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 antibody (rC81/3442) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-rC81-3442-Flow-Cytometry-NBP3-07474-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody [NBP1-90329] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90329-img0008.jpg "Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody [NBP1-90329] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP1-90329] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-small-subunit-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90329-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human spleen using AIF-1/Iba1 Antibody shows very strong membranous positivity in cells in red pulp.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human cerebral cortex using AIF-1/Iba1 Antibody shows strong membranous positivity in glial cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human kidney using AIF-1/Iba1 Antibody shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human lymphoid tissues using AIF-1/Iba1 Antibody shows very strong membranous positivity in non-germinal, and germinal center cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human placenta using AIF-1/Iba1 Antibody shows very strong cytoplasmic positivity in Hofbauer cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human skeletal muscle using AIF-1/Iba1 Antibody shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAM107A Antibody [NBP2-30589] - Staining of human cell line HUVEC TERT2 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30589-img0007.jpg "Immunocytochemistry/Immunofluorescence: FAM107A Antibody [NBP2-30589] - Staining of human cell line HUVEC TERT2 shows localization to nuclear speckles. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining in human fallopian tube and liver tissues . Corresponding FAM107A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0013.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human cerebellum shows moderate to strong positivity.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human fallopian tube shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0009.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human hippocampus shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0010.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0011.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human prostate shows moderate nuclear positivity.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0012.jpg)
![Western Blot: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 by WB of HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 lysate. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-BLR042F-Western-Blot-NBP2-76421-img0003.jpg "Western Blot: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 by WB of HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 lysate. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry/Immunofluorescence: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 (red) in FFPE breast carcinoma by IHC-IF. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-BLR042F-Immunocytochemistry-Immunofluorescence-NBP2-76421-img0004.jpg)
![Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinantmonoclonal antibody [BLR042F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-BLR042F-Immunohistochemistry-NBP2-76421-img0002.jpg)
![Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/CD134 in FFPE lung carcinoma. . Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (NBP2-76421). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-BLR042F-Immunohistochemistry-NBP2-76421-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: OX40/TNFRSF4 Antibody (BLR042F) [NBP2-76421] - Detection of human OX40/TNFRSF4 by WB of immunoprecipitates from MJ lysate. Antibodies: Rabbit anti-OX40/TNFRSF4 recombinant monoclonal [BLR042F] (NBP2-76421) and other rabbit anti-OX40/TNFRSF4 recombinant monoclonal antibody.](http://images.novusbio.com/fullsize/OX40-TNFRSF4-Antibody-BLR042F-Immunoprecipitation-NBP2-76421-img0005.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)