| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92907-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42210SS | |||
| Azide and BSA Free | NBP2-80739 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87791-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44198 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39055-25ul | |||
![Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Western-Blot-NBP3-15662-img0003.jpg "Western Blot: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Analysis of extracts of various cell lines, using p27/Kip1 antibody (NBP3-15662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (9R5B4) [NBP3-15662] - Rat ovary using p27/Kip1 Rabbit mAb (NBP3-15662) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-9R5B4-Immunohistochemistry-Paraffin-NBP3-15662-img0001.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517315585.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734546.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251734555.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517333867.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517304988.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-5220251731107.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517363713.jpg)
![Immunohistochemistry: p27/Kip1 Antibody (9R5B4) [p27/Kip1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using p27/Kip1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15662_rabbit-p27-kip1-mab-9r5b4-52202517311074.jpg)
![Western Blot: CSN1 Antibody [NBP2-92907] - Analysis of extracts of various cell lines, using CSN1 .Exposure time: 90s.](http://images.novusbio.com/fullsize/CSN1-Antibody-Western-Blot-NBP2-92907-img0005.jpg "Western Blot: CSN1 Antibody [NBP2-92907] - Analysis of extracts of various cell lines, using CSN1 .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: CSN1 Antibody [NBP2-92907] - Immunofluorescence analysis of U2OS cells using CSN1 Rabbit pAb (NBP2-92907) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92907-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CSN1 Antibody [NBP2-92907] - Immunofluorescence analysis of C6 cells using CSN1 Rabbit pAb (NBP2-92907) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CSN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92907-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: CSN1 Antibody - BSA Free [NBP2-92907] - Immunofluorescence analysis of NIH/3T3 cells using CSN1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-42202514275224.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-4220251431816.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-42202514251852.jpg)
![Immunohistochemistry: CSN1 Antibody - BSA Free [NBP2-92907] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CSN1 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-92907_rabbit-polyclonal-csn1-antibody-4220251429067.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis on K562 cell lysate using anti-JAK2 mouse mAb.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Western-Blot-NBP2-66913-img0003.jpg "Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis on K562 cell lysate using anti-JAK2 mouse mAb.")
![Immunocytochemistry/Immunofluorescence: Jak2 Antibody (6-D3) [NBP2-66913] - Staining JAK2 in HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Immunocytochemistry-Immunofluorescence-NBP2-66913-img0001.jpg)
![Immunohistochemistry-Paraffin: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- JAK2 mouse mAb.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Immunohistochemistry-Paraffin-NBP2-66913-img0002.jpg)
![Microarray: Jak2 Antibody (6-D3) [NBP2-66913] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Jak2-Antibody-6-D3-Microarray-NBP2-66913-img0004.jpg)
![Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] -Analysis on K562 cell lysates using anti-JAK2 mouse mAb.](http://images.novusbio.com/fullsize/nbp2-66913_mouse-monoclonal-jak2-antibody-6-d3-western-blot-187202411641..jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Western-Blot-NBP2-42210-img0001.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Immunohistochemistry-Paraffin-NBP2-42210-img0003.jpg)
![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Flow-Cytometry-NBP2-42210-img0002.jpg)
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Western-Blot-NBP1-87791-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human cell line A549 shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87791-img0006.jpg)
![Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-341](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Western-Blot-NBP1-87791-img0004.jpg)
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0008.jpg)
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human testis shows low expression as expected.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0009.jpg)
![Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Western-Blot-NBP2-88912-img0007.jpg "Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunocytochemistry-Immunofluorescence-NBP2-88912-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human endometrium shows strong nuclear positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human liver cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0005.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Analysis in human placenta and kidney tissues using NBP2-88911 antibody. Corresponding CDK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunohistochemistry-Paraffin-NBP2-88911-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Analysis in human placenta and kidney tissues using NBP2-88911 antibody. Corresponding CDK2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CDK2 Antibody (CL7990) [NBP2-88911] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Western-Blot-NBP2-88911-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of SK-MEL-30 cells using the Anti-CDK2 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunocytochemistry-Immunofluorescence-NBP2-88911-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunohistochemistry-Paraffin-NBP2-88911-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunohistochemistry-Paraffin-NBP2-88911-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunohistochemistry-Paraffin-NBP2-88911-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDK2-Antibody-CL7990-Immunohistochemistry-Paraffin-NBP2-88911-img0005.jpg)
![Western Blot: EML4 Antibody [NBP2-39055] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HepG2](http://images.novusbio.com/fullsize/EML4-Antibody-Western-Blot-NBP2-39055-img0002.jpg "Western Blot: EML4 Antibody [NBP2-39055] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HepG2")
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0004.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining in human appendix and cerebral cortex tissues using anti-EML4 antibody. Corresponding EML4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP2-39055 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP2-39055] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP2-39055-img0009.jpg)
![Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42509-img0003.jpg "Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Staining of paraffin embedded Human Kidney with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518274621.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518431912.jpg)