| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03045-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59444-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03298-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59468-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97603-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
![Western Blot: CDK2 Antibody [NBP3-03045] - Analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/CDK2-Antibody-Knockout-Validated-NBP3-03045-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CDK2 Antibody [NBP3-03045] - Analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using CDK2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: CDK2 Antibody - Azide and BSA Free [CDK2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] CDK2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03045_rabbit-polyclonal-cdk2-antibody-522025163631.jpg)
![Western Blot: p27/Kip1 Antibody (7B9) [NBP2-59444] - Lane 1: MCF-7 cell lysates, Lane 2: NIH/3T3 cell lysates](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-7B9-Western-Blot-NBP2-59444-img0001.jpg "Western Blot: p27/Kip1 Antibody (7B9) [NBP2-59444] - Lane 1: MCF-7 cell lysates, Lane 2: NIH/3T3 cell lysates")
![Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (7B9) [NBP2-59444] - Analysis of CDKN1b in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDKN1b antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-7B9-Immunocytochemistry-Immunofluorescence-NBP2-59444-img0002.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody [NBP3-03298] - Analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using p18INK4c/CDKN2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking Buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/p18INK4c-CDKN2C-Antibody-Knockout-Validated-NBP3-03298-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p18INK4c/CDKN2C Antibody [NBP3-03298] - Analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using p18INK4c/CDKN2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking Buffer: 3% nonfat dry milk in TBST.")
![Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Western-Blot-NBP2-59468-img0001.jpg "Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Analysis of PA2G4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of PA2G4 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-59468-img0002.jpg)
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0001.jpg "Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of various cell lines, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: Cyclin D3 Antibody (4C5C8) [NBP3-16309] - Western blot analysis of extracts of Rat lung, using Cyclin D3 Rabbit mAb (NBP3-16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-4C5C8-Western-Blot-NBP3-16309-img0002.jpg)
![Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/RRM1-Antibody-4P4U1-Western-Blot-NBP3-16364-img0001.jpg "Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: RRM1 Antibody (4P4U1) [NBP3-16364] - Western blot analysis of extracts of Rat lung, using RRM1 Rabbit mAb (NBP3-16364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RRM1-Antibody-4P4U1-Western-Blot-NBP3-16364-img0002.jpg)
![Western Blot: CDC25B Antibody (4D5S10) [NBP3-16877] - Western blot analysis of extracts of various cell lines, using CDC25B Rabbit mAb (NBP3-16877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CDC25B-Antibody-4D5S10-Western-Blot-NBP3-16877-img0002.jpg "Western Blot: CDC25B Antibody (4D5S10) [NBP3-16877] - Western blot analysis of extracts of various cell lines, using CDC25B Rabbit mAb (NBP3-16877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: CDC25B Antibody (4D5S10) [NBP3-16877] - Immunohistochemistry of paraffin-embedded human appendix using CDC25B Rabbit mAb (NBP3-16877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDC25B-Antibody-4D5S10-Immunohistochemistry-Paraffin-NBP3-16877-img0001.jpg)
![Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-97603-img0003.jpg "Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-97603] - Immunofluorescence staining of NCAPH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97603 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97603_rabbit-polyclonal-ncaph-antibody-23520251433150.png)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![SMC5 Antibody [NBP3-12159] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SMC5-Antibody-NBP3-12159-img0001.jpg "SMC5 Antibody [NBP3-12159] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![SMC5 Antibody [NBP3-12159] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/SMC5-Antibody-NBP3-12159-img0002.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98433 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98433_rabbit-polyclonal-e-cadherin-antibody-23520252135138.png)