| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92161-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24593-25ul | |||
| Azide and BSA Free | NBP3-43862 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14016 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24594-25ul | |||
| Azide and BSA Free | NBP3-43863 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48690-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24592-25ul | |||
| Azide and BSA Free | NBP3-43860 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03978SS | |||
![Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Western-Blot-NBP2-24593-img0001.jpg "Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24593] - Formalin-fixed, paraffin-embedded human spleen stained with 14-3-3 zeta antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP2-24593-img0002.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0005.jpg "Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunocytochemistry/Immunofluorescence: HBO1 Antibody [NBP1-92161] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92161-img0007.jpg)
![Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161] - Staining of human stomach shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunohistochemistry-Paraffin-NBP1-92161-img0002.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0004.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92161_rabbit-polyclonal-hbo1-antibody-295202516464821.jpg)
![Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunocytochemistry-Immunofluorescence-NBP3-13982-img0002.jpg "Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0004.jpg)
![Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Flow cytometric analysis of PFA-fixed HeLa cells. NFIA antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Flow-Cytometry-NBP3-13982-img0001.jpg)
![Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human lung stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Immunohistochemistry-Paraffin-NBP3-13982-img0003.jpg)
![Protein Array: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NFIA Antibody (PCRP-NFIA-2C6).](http://images.novusbio.com/fullsize/NFIA-Antibody-PCRP-NFIA-2C6-Protein-Array-NBP3-13982-img0006.jpg)
![Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining in human bone marrow and pancreas tissues using anti-BIN2 antibody. Corresponding BIN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining in human bone marrow and pancreas tissues using anti-BIN2 antibody. Corresponding BIN2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining of human bone marrow, liver, lymph node and pancreas using Anti-BIN2 antibody NBP2-48690 (A) shows similar protein distribution across tissues to independent antibody NBP2-48691 (B).](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0006.jpg)
![Western Blot: BIN2 Antibody [NBP2-48690] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/BIN2-Antibody-Western-Blot-NBP2-48690-img0002.jpg)
![Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining of human liver.](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0008.jpg)
![Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0003.jpg)
![Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0004.jpg)
![Immunohistochemistry-Paraffin: BIN2 Antibody [NBP2-48690] - Staining of human lymph node.](http://images.novusbio.com/fullsize/BIN2-Antibody-Immunohistochemistry-Paraffin-NBP2-48690-img0007.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CtIP-Antibody-Immunoprecipitation-NB100-79809-img0003.jpg "Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Western-Blot-NBP1-31325-img0005.jpg "Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0006.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0003.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242013121.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242075967.jpg)
![Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser318]-Antibody-Western-Blot-NBP2-03978-img0003.jpg "Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.")
![Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: SNF5 Antibody [NB100-57525] - Detection of Human and Mouse SMARCB1/SNF5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0011.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SNF5 Antibody [NB100-57525] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-57525 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCB1/SNF5 was also immunoprecipitated by another rabbit anti-SMARCB1/SNF5 antibody, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/SNF5-Antibody-Western-Blot-NB100-57525-img0002.jpg)
![Immunohistochemistry: SNF5 Antibody [NB100-57525] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunohistochemistry-NB100-57525-img0010.jpg)
![Immunoprecipitation: SNF5 Antibody [NB100-57525] - Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody NB100-57525 (lot NB100-57525-2) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody NB100-57525-1. For blotting immunoprecipitated SMARCB1/SNF5, NB100-57525 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SNF5-Antibody-Immunoprecipitation-NB100-57525-img0014.jpg)