| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89498-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98962-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52683-0.025mg | |||
![Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-288202412442975.jpg "Western Blot: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32782) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HT-29 treated with 0.5uM Doxorubicin for 24 hours cell lysate Lane 3: HT-29 treated with 0.5uM Doxorubicin for 24 hours, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32782) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 [p Ser15] Antibody (PSH01-98) [NBP3-32782] - Immunocytochemistry analysis of HT-29 treated with or without 0.5μM Doxorubicin for 24 hours cells labeling p53 with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32782) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32782_rabbit-p53-p-ser15-mab-psh01-98-29202410234274.jpg)
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0005.jpg "Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).")
![Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.](http://images.novusbio.com/fullsize/p53-p-Ser392-Antibody-FP3-2-FPS392-Immunohistochemistry-NB500-575-img0004.jpg)
![Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-048-Flow-Cytometry-NBP2-89498-img0001.jpg "Flow Cytometry: VE-Cadherin Antibody (048) [NBP2-89498] - Analysis of Human CD144 expression on HUVEC cells. Cells were stained with purified anti-Human CD144, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: VE-Cadherin Antibody (048) [NBP2-89498] - Staining of CDH5 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89498 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89498_rabbit-monoclonal-ve-cadherin-antibody-048-235202521451786.png)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32142) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32142) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32142_rabbit-cd30-tnfrsf8-mab-psh04-10-2782024163167.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32142) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32142) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-10) [NBP3-32142] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32142) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32142) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32142_rabbit-cd30-tnfrsf8-mab-psh04-10-29820241433362.jpg)
![Western Blot: Somatostatin R1/SSTR1 Antibody [NBP2-88331] - WB Suggested Anti-SSTR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Western-Blot-NBP2-88331-img0002.jpg "Western Blot: Somatostatin R1/SSTR1 Antibody [NBP2-88331] - WB Suggested Anti-SSTR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate")
![Immunohistochemistry: Somatostatin R1/SSTR1 Antibody [NBP2-88331] - Immunohistochemistry with Pancreas tissue at an antibody concentration of 5ug/ml using anti-SSTR1 antibody](http://images.novusbio.com/fullsize/Somatostatin-R1-SSTR1-Antibody-Immunohistochemistry-NBP2-88331-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP2-98962] - Immunochemical staining of human Cyclin B2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP2-98962-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP2-98962] - Immunochemical staining of human Cyclin B2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP2-98962] - Immunofluorescence staining of Cyclin B2 in SW480 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98962 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98962_rabbit-polyclonal-cyclin-b2-antibody-23520251633503.png)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98433 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98433_rabbit-polyclonal-e-cadherin-antibody-23520252135138.png)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![SDS-Page: Recombinant Human Endothelin-1 Protein [H00001906-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Endothelin-1-Protein-SDS-Page-H00001906-Q01-img0002.jpg "SDS-Page: Recombinant Human Endothelin-1 Protein [H00001906-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: EB3 Antibody (KT53) [NBP3-23293] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23293_rat-eb3-mab-kt53-199202312523647.jpg "Western Blot: EB3 Antibody (KT53) [NBP3-23293] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-4) - Chimeric [NBP2-52683] - Flow-cytometry using anti-CD30 antibody Ki-4. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ki-4 (NBP2-52683) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-4-Chimeric-Flow-Cytometry-NBP2-52683-img0001.jpg "Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-4) - Chimeric [NBP2-52683] - Flow-cytometry using anti-CD30 antibody Ki-4. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ki-4 (NBP2-52683) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (Ki-1/1747R) [NBP2-54556] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD30 Recombinant Rabbit Monoclonal Antibody (Ki-1/1747R).](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-1-1747R-Immunohistochemistry-Paraffin-NBP2-54556-img0001.jpg "Immunohistochemistry-Paraffin: CD30/TNFRSF8 Antibody (Ki-1/1747R) [NBP2-54556] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD30 Recombinant Rabbit Monoclonal Antibody (Ki-1/1747R).")