| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76498-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03535-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94511-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94811-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: CtIP Antibody (19E8) [NBP1-41205] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-41205_mouse-monoclonal-ctip-antibody-19e8-1610202419414519.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CtIP Antibody (19E8) [NBP1-41205] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CtIP antibody [19E8] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>CtIP antibody [19E8] detects CtIP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CtIP protein stained by CtIP antibody [19E8] (NBP1-41205) diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/nbp1-41205_mouse-monoclonal-ctip-antibody-19e8-immunocytochemistry-immunofluorescence-261120251974522.jpg)
![Western Blot: SGTA Antibody (2E11) [H00006449-M06] - SGTA expression in PC-12 ( Cat # L012V1 ).](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Western-Blot-H00006449-M06-img0002.jpg "Western Blot: SGTA Antibody (2E11) [H00006449-M06] - SGTA expression in PC-12 ( Cat # L012V1 ).")
![Immunocytochemistry/Immunofluorescence: SGTA Antibody (2E11.) [H00006449-M06] - SGTA Antibody (2E11) [H00006449-M06] - Analysis of monoclonal antibody to SGTA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Immunocytochemistry-Immunofluorescence-H00006449-M06-img0008.jpg)
![Western Blot: SGTA Antibody (2E11) [H00006449-M06] - SGTA expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Western-Blot-H00006449-M06-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SGTA Antibody (2E11) [H00006449-M06] - Analysis of monoclonal antibody to SGTA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Immunocytochemistry-Immunofluorescence-H00006449-M06-img0004.jpg)
![Sandwich ELISA: SGTA Antibody (2E11) [H00006449-M06] - Detection limit for recombinant GST tagged SGTA is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Sandwich-ELISA-H00006449-M06-img0007.jpg)
![Immunoprecipitation: SGTA Antibody (2E11) [H00006449-M06] - Analysis of SGTA transfected lysate using anti-SGTA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGTA rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SGTA-Antibody-2E11-Immunoprecipitation-H00006449-M06-img0006.jpg)
![Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SLC39A1-Antibody-Western-Blot-NBP1-76498-img0001.jpg "Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SLC39A1 Antibody [NBP1-76498] - Human kidney tissue at 2.5 ug/mL. dilution](http://images.novusbio.com/fullsize/SLC39A1-Antibody-Immunohistochemistry-NBP1-76498-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SLC39A1 Antibody - BSA Free [NBP1-76498] - Immunofluorescence of SLC39A1 in human kidney tissue with SLC39A1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76498_rabbit-polyclonal-slc39a1-antibody-2711202420272442.jpg)
![Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.](http://images.novusbio.com/fullsize/LRDD-Antibody-Anto-1-Western-Blot-NBP1-97595-img0001.jpg "Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.")
![Immunohistochemistry-Paraffin: LRDD Antibody (Anto-1) [NBP1-97595] - Staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1). Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.](http://images.novusbio.com/fullsize/LRDD-Antibody-Anto-1-Immunohistochemistry-Paraffin-NBP1-97595-img0002.jpg)
![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-20356-img0004.jpg "Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30482-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Western-Blot-NBP1-30482-img0008.jpg)
![Immunoprecipitation: Topoisomerase I Antibody [NBP1-30482] - Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 (lot NBP1-30482-3) used for IP at 6 ug per reaction. TOP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30482-2). For blotting immunoprecipitated TOP1, NBP1-30482 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-Immunoprecipitation-NBP1-30482-img0012.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03535_rabbit-polyclonal-lrdd-antibody-52202516334114.jpg "Immunocytochemistry/ Immunofluorescence: PIDD Antibody - BSA Free [PIDD] - Immunofluorescence analysis of HeLa cells using PIDD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded mouse brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0004.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded human lung cancer using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0002.jpg)
![Immunohistochemistry-Paraffin: LRDD Antibody [NBP3-03535] - Paraffin-embedded rat brain using LRDD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/LRDD-Antibody-Immunohistochemistry-Paraffin-NBP3-03535-img0003.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Western-Blot-NBP2-94511-img0001.jpg "Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of SH-SY5Y cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Mouse brain using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-94511-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of Neuro-2a cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94511-img0003.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Western blot analysis of extracts of various cell lines, using Muscarinic Acetylcholine Receptor M1/CHRM1 antibody (NBP2-94811) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Western-Blot-NBP2-94811-img0006.jpg "Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Western blot analysis of extracts of various cell lines, using Muscarinic Acetylcholine Receptor M1/CHRM1 antibody (NBP2-94811) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of C6 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of NIH-3T3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of OVCAR-3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94811] - Analysis of OVCAR-3 cells using Muscarinic Acetylcholine Receptor M1/CHRM1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94811-img0003.jpg)