| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56736SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49550-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89461-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41302-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38226-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61735-0.025ml | |||
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-Western-Blot-NB100-56736-img0002.jpg "Western Blot: RGS2 Antibody [NB100-56736] - Western bot analysis of RGS2 in human heart lysate using RGS2 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-49550] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-49550-img0004.jpg "Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-49550] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-49550] - Staining of human thyroid gland shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-49550-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-49550] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-49550-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-49550] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-49550-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0010.jpg)
![RGS2 antibody [HL2988] detects RGS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.RGS2 stained by RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48758_rabbit-rgs2-mab-hl2988-immunohistochemistry-paraffin-1411202518252010.jpg "<b>Orthogonal Strategies Validation. </b>RGS2 antibody [HL2988] detects RGS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.RGS2 stained by RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Whole tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48758_rabbit-rgs2-mab-hl2988-western-blot-141120251842172.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48758_rabbit-rgs2-mab-hl2988-western-blot-141120251838178.jpg)
![RGS2 antibody [HL2988] detects RGS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.RGS2 stained by RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48758_rabbit-rgs2-mab-hl2988-immunohistochemistry-paraffin-1411202518435624.jpg)
![RGS2 antibody [HL2988] detects RGS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.RGS2 stained by RGS2 antibody [HL2988] (NBP3-48758) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48758_rabbit-rgs2-mab-hl2988-immunohistochemistry-paraffin-141120251842527.jpg)
![Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SUV39H2-Antibody-Western-Blot-NB100-1140-img0003.jpg "Western Blot: SUV39H2 Antibody [NB100-1140] - staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry: SUV39H2 Antibody [NB100-1140] - Dermal Microvascular Endothelial Cells were fixed with 4% Paraformaldehyde followed by immunofluorescence staining using 1:100 dilution of pAb. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/SUV39H2-Antibody-Immunocytochemistry-NB100-1140-img0005.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32186-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32186-img0003.jpg)
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32186 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32186-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP1-89461] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89461-img0006.jpg "Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP1-89461] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DRG2 Antibody [NBP1-89461] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89461-img0007.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Immunocytochemistry-Immunofluorescence-NBP3-13617-img0002.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0003.jpg)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13617_mouse-hsp27-p-ser-82-mab-gt357-western-blot-2611202518484816.jpg)
![Western Blot: SESN2 Antibody [NBP2-41302] - Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SESN2-Antibody-Western-Blot-NBP2-41302-img0001.jpg "Western Blot: SESN2 Antibody [NBP2-41302] - Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SESN2 Antibody - BSA Free [NBP2-41302] - Immunohistochemistry of SESN2 in mouse kidney tissue with SESN2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41302_rabbit-polyclonal-sesn2-antibody-2711202420405586.jpg)
![Immunocytochemistry/ Immunofluorescence: SESN2 Antibody - BSA Free [NBP2-41302] - Immunofluorescence of SESN2 in mouse kidney tissue with SESN2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41302_rabbit-polyclonal-sesn2-antibody-2711202419381733.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415111899.jpg "Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: CIRBP Antibody [NBP3-38226] - Western blot analysis of lysates from Rat testis, using CIRBP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121323.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Human placenta using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202415121388.jpg)
![Immunohistochemistry: CIRBP Antibody [NBP3-38226] - Immunohistochemistry analysis of paraffin-embedded Rat brain using CIRBP Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38226_rabbit-cirbp-pab-912202414163069.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0004.jpg "Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against human CIRBP (AA: 1-90) recombinant protein. (Expected MW is 35.9 kDa)")
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded bladder cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0003.jpg)
![Western Blot: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis using CIRBP mAb against HEK293 (1) and CIRBP (AA: 1-90)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Western-Blot-NBP2-61735-img0005.jpg)
![Immunohistochemistry: CIRBP Antibody (6E12D5) [NBP2-61735] - Analysis of paraffin-embedded colon cancer tissues using CIRBP mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-Immunohistochemistry-NBP2-61735-img0002.jpg)
![ELISA: CIRBP Antibody (6E12D5) [NBP2-61735] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CIRBP-Antibody-6E12D5-ELISA-NBP2-61735-img0001.jpg)