| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76698-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61768-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47861-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48089 | |||
| Azide and BSA Free | NBP2-86682 | |||
![Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17(IL-17A+IL-17B) protein per lane. Antibodies: IL-27 NBP1-76698, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-27 Antibody [NBP1-76698] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: IL-27 NBP1-76698 (4 ug/mL), IL-27 NBP1-76412 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-27-Antibody-Western-Blot-NBP1-76698-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-27 Antibody - BSA Free [NBP1-76698] - Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76698_rabbit-polyclonal-il-27-antibody-2711202420212370.jpg)
![Western Blot: Cullin 2 Antibody [NBP1-49873] - Analysis of Cullin 2 in NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-49873 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cullin-2-Antibody-Western-Blot-NBP1-49873-img0002.jpg "Western Blot: Cullin 2 Antibody [NBP1-49873] - Analysis of Cullin 2 in NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-49873 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Cullin 2 Antibody [NBP1-49873] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Cullin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-49873-img0003.jpg)
![Western Blot: ARF6 Antibody [NBP1-58310] - Titration: 0.2-1ug/ml, Positive Control: MCF7 cell Lysate](http://images.novusbio.com/fullsize/ARF6-Antibody-Western-Blot-NBP1-58310-img0009.jpg "Western Blot: ARF6 Antibody [NBP1-58310] - Titration: 0.2-1ug/ml, Positive Control: MCF7 cell Lysate")
![Immunohistochemistry-Paraffin: ARF6 Antibody [NBP1-58310] - Human Brain Tissue (Cerebellum), antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/ARF6-Antibody-Immunohistochemistry-Paraffin-NBP1-58310-img0007.jpg)
![Western Blot: ARF6 Antibody [NBP1-58310] - Titration: 2 ug/ml Positive Control: Human NT-2 cells and Mouse WT brain.](http://images.novusbio.com/fullsize/ARF6-Antibody-Western-Blot-NBP1-58310-img0008.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0004.jpg "Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0005.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0001.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0002.jpg)
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)
![Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/IL-6-Antibody-1-6-Western-Blot-NBP2-89149-img0001.jpg "Western Blot: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: IL-6 Antibody (1-6) [NBP2-89149] - Analysis of EL4 cells labeling IL-6 with Rabbit anti-IL-6 antibody at 1/1,000 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-6 antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-89149_mouse-monoclonal-il-6-antibody-1-6-immunocytochemistry-immunofluorescence-266202412753..jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0003.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0001.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) - IHC-Prediluted [NBP2-48319] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48319-img0002.jpg)
![Western Blot: SA1 Antibody (8810) [NBP2-43750] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [8810].](http://images.novusbio.com/fullsize/SA1-Antibody-8810-Western-Blot-NBP2-43750-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SA1 Antibody (8810) [NBP2-43750] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [8810].")
![<b>Genetic Strategies Validation. </b>Western Blot: SA1 Antibody (8810) [NBP2-43750] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with STAG1 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/SA1-Antibody-8810-Western-Blot-NBP2-43750-img0001.jpg)
![SA1 antibody [GT8810] detects SA1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA1 protein stained by SA1 antibody [GT8810] (NBP2-43750) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43750_mouse-monoclonal-sa1-antibody-8810-immunocytochemistry-immunofluorescence-261120251829010.jpg)
![Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).](http://images.novusbio.com/fullsize/c-Myc-Antibody-SPM237-Immunohistochemistry-Paraffin-NBP2-45145-img0001.jpg "Immunohistochemistry-Paraffin: c-Myc Antibody (SPM237) [NBP2-45145] - Human Cervical Carcinoma stained with c-myc Monoclonal Antibody (SPM237).")
![Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SRPK1-Antibody-Western-Blot-NBP1-30108-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- SRPK1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SRPK1-Antibody-Immunohistochemistry-NBP1-30108-img0002.jpg)
![Western Blot: p190RhoGAP Antibody [NB100-88154] - FAK and p190RhoGAP are inactivated by GT. Western blot analysis of total extracts of BEAS-2B cells either non-treated (NT) or treated with 50 uM of FAK14 (g) for 30 min to 4 h. The inhibitor caused FAK and p190RhoGAP dephosphorylation and MKK4, JNK and Bim phosphorylations. Tubulin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05850-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Western-Blot-NB100-88154-img0008.jpg "Western Blot: p190RhoGAP Antibody [NB100-88154] - FAK and p190RhoGAP are inactivated by GT. Western blot analysis of total extracts of BEAS-2B cells either non-treated (NT) or treated with 50 uM of FAK14 (g) for 30 min to 4 h. The inhibitor caused FAK and p190RhoGAP dephosphorylation and MKK4, JNK and Bim phosphorylations. Tubulin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05850-w), licensed under a CC-BY license.")
![Western Blot: p190RhoGAP Antibody [NB100-88154] - Whole cell lysate (50 ug) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Western-Blot-NB100-88154-img0006.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p190RhoGAP Antibody [NB100-88154] - Western Blot and Immunoprecipitation: p190RhoGAP Antibody NB100-88154-Detection of Human p190RhoGAP on HeLa whole cell lysate using NB100-88154. Gp190RhoGAP was also immunoprecipitated by another rabbit ap190RhoGAP antibody.](http://images.novusbio.com/fullsize/p190RhoGAP-Antibody-Western-Blot-NB100-88154-img0007.jpg)