| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80701-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90196-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-51826SS | |||
| Azide and BSA Free | NBP2-80808 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70183 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37999-20ul | |||
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0016.jpg "Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Immunohistochemistry-Paraffin-H00001445-M01-img0010.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0012.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3 Analysis of CSK expression in HL-60.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0014.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3. Analysis of CSK expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0015.jpg)
![Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-Western-Blot-H00001445-M01-img0017.jpg)
![ELISA: CSK Antibody (3A3) [H00001445-M01] - Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CSK-Antibody-3A3-ELISA-H00001445-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Analysis in human testis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP1-80701-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Analysis in human testis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD81 Antibody [NBP1-80701] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80701-img0004.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP1-80701-img0010.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP1-80701-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP1-80701-img0008.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP1-80701] - Staining of human prostate shows weak to moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP1-80701-img0009.jpg)
![Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Western-Blot-NBP1-84928-img0012.jpg "Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: MAPRE1 Antibody [NBP1-84928] - Staining of human cell line U-251 MG shows localization to cytosol and microtubule organizing center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84928-img0019.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining in human testis and skeletal muscle tissues using anti-MAPRE1 antibody. Corresponding MAPRE1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84928-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Western-Blot-NBP1-84928-img0011.jpg)
![Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84928-img0016.jpg)
![Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/MAPRE1-Antibody-Immunohistochemistry-Paraffin-NBP1-84928-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90196-img0006.jpg "Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-90196] - Staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Western Blot: AKAP95/AKAP8 Antibody [NBP1-90196] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/AKAP95-AKAP8-Antibody-Western-Blot-NBP1-90196-img0005.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0010.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0004.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0005.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0008.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human placenta shows no cytoplasmic positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0009.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0011.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84323 (A) shows similar protein distribution across tissues to independent antibody NBP1-84322 (B).](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0027.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84323 (A) shows similar protein distribution across tissues to independent antibody NBP1-84322 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84323] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84323-img0019.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0030.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0025.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0028.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84323] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84323-img0029.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Human colon cancer. APC stained by APC antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NBP2-15422-img0009.jpg "Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Human colon cancer. APC stained by APC antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min.")
![Immunohistochemistry-Paraffin: APC Antibody [NBP2-15422] - Rat colon. APC antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NBP2-15422-img0006.jpg)
![Western Blot: APC Antibody [NBP2-15422] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15422_rabbit-polyclonal-apc-antibody-910202420115060.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) unboiled HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (NBP2-15422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15422_rabbit-polyclonal-apc-antibody-western-blot-2611202518381518.jpg)
![Flow Cytometry: IL-3 Antibody (MP2-8F8) [NBP1-51826] - Analysis of IL-3 concentration.](http://images.novusbio.com/fullsize/IL-3-Antibody-MP2-8F8-Flow-Cytometry-NBP1-51826-img0001.jpg "Flow Cytometry: IL-3 Antibody (MP2-8F8) [NBP1-51826] - Analysis of IL-3 concentration.")
![Western Blot: APC Antibody (2B8) [NBP2-46529] - Human recombinant protein fragment corresponding to amino acids 955-1382 of human APC (NP_001120983) produced in E.coli.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Western-Blot-NBP2-46529-img0007.jpg "Western Blot: APC Antibody (2B8) [NBP2-46529] - Human recombinant protein fragment corresponding to amino acids 955-1382 of human APC (NP_001120983) produced in E.coli.")
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0006.jpg)
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0001.jpg)
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Adenocarcinoma of Human colon.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0002.jpg)
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0003.jpg)
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0004.jpg)
![Immunohistochemistry: APC Antibody (2B8) [NBP2-46529] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/APC-Antibody-2B8-Immunohistochemistry-NBP2-46529-img0005.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP3-37999] - Immunofluorescence analysis of U-2 OS cells using ILF3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37999_rabbit-ilf3-pab-10122024121040.jpg "Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP3-37999] - Immunofluorescence analysis of U-2 OS cells using ILF3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP3-37999] - Immunofluorescence analysis of C6 cells using ILF3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37999_rabbit-ilf3-pab-10122024121065.jpg)
![Immunoprecipitation: ILF3 Antibody [NBP3-37999] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug ILF3 antibody. Western blot was performed from the immunoprecipitate using ILF3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-37999_rabbit-ilf3-pab-10122024121042.jpg)
![Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP3-37999] - Immunofluorescence analysis of NIH-3T3 cells using ILF3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37999_rabbit-ilf3-pab-1012202411531074.jpg)
![Western Blot: ILF3 Antibody [NBP3-37999] - Western Blot analysis of lysates from Hep G2 cells using ILF3 Rabbit pAbat 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit.Exposuretime:0.5s.](http://images.novusbio.com/fullsize/nbp3-37999_rabbit-ilf3-pab-3112025177028.jpg%3Ft=1702607471)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Western-Blot-NBP1-97587-img0006.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Flow-Cytometry-NBP1-97587-img0004.jpg)