| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03736-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13920-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76517-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76505-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03712-20ul | |||
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48755_rabbit-flightless-i-mab-hl2966-western-blot-141120251837278.jpg "Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Flightless I antibody [HL2966] detects Flightless I protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Flightless I stained by Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48755_rabbit-flightless-i-mab-hl2966-immunohistochemistry-paraffin-1411202518324122.jpg)
![Flightless I antibody [HL2966] detects Flightless I protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Flightless I stained by Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48755_rabbit-flightless-i-mab-hl2966-immunocytochemistry-immunofluorescence-1411202518372718.jpg)
![Flightless I antibody [HL2966] detects Flightless I protein by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Flightless I stained by Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48755_rabbit-flightless-i-mab-hl2966-immunohistochemistry-paraffin-141120251835463.jpg)
![Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Western-Blot-NBP3-03736-img0002.jpg "Western Blot: Exostosin 1 Antibody [NBP3-03736] - Analysis of extracts of various cell lines, using Exostosin 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of NIH/3T3 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-1620238270..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of PC-12 cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202382850..jpg)
![Immunocytochemistry/Immunofluorescence: Exostosin 1 Antibody [NBP3-03736] -Analysis of U2OS cells using EXT1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-immunocytochemistry-immunofluorescence-16202383040..jpg)
![Immunocytochemistry/ Immunofluorescence: Exostosin 1 Antibody - Azide and BSA Free [NBP3-03736] - Immunofluorescence analysis of HeLa cells using Exostosin 1 Rabbit pAb (A2030) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03736_rabbit-polyclonal-exostosin-1-antibody-52202519313480.jpg)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in NIH/3T3.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Western-Blot-H00002253-M01-img0009.jpg "Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in NIH/3T3.")
![Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in PC-12.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Western-Blot-H00002253-M01-img0005.jpg)
![Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10 Analysis of FGF8 expression in HepG2.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Western-Blot-H00002253-M01-img0006.jpg)
![Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Raw 264.7.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Western-Blot-H00002253-M01-img0007.jpg)
![Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Jurkat.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Western-Blot-H00002253-M01-img0008.jpg)
![Sandwich ELISA: FGF-8 Antibody (2A10) [H00002253-M01] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FGF-8-Antibody-2A10-Sandwich-ELISA-H00002253-M01-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DHX16 Antibody [NBP2-13920] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DHX16-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13920-img0003.jpg "Immunocytochemistry/Immunofluorescence: DHX16 Antibody [NBP2-13920] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining in human testis and liver tissues using anti-DHX16 antibody. Corresponding DHX16 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DHX16-Antibody-Immunohistochemistry-Paraffin-NBP2-13920-img0006.jpg)
![Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining of human kidney shows strong nuclear positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/DHX16-Antibody-Immunohistochemistry-Paraffin-NBP2-13920-img0001.jpg)
![Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/DHX16-Antibody-Immunohistochemistry-Paraffin-NBP2-13920-img0004.jpg)
![Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/DHX16-Antibody-Immunohistochemistry-Paraffin-NBP2-13920-img0005.jpg)
![Western Blot: ZBTB4 Antibody [NBP1-76517] - SK-N-SH cell lysate with ZBTB4 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ZBTB4-Antibody-Western-Blot-NBP1-76517-img0001.jpg "Western Blot: ZBTB4 Antibody [NBP1-76517] - SK-N-SH cell lysate with ZBTB4 antibody at 1 ug/mL.")
![Immunohistochemistry: ZBTB4 Antibody - BSA Free [NBP1-76517] - Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76517_rabbit-polyclonal-zbtb4-antibody-271120241947587.jpg)
![Immunocytochemistry/ Immunofluorescence: ZBTB4 Antibody - BSA Free [NBP1-76517] - Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76517_rabbit-polyclonal-zbtb4-antibody-2711202420405576.jpg)
![Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Western-Blot-NBP3-16650-img0003.jpg "Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Confocal imaging of HeLa cells using CAD Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16650_rabbit-cad-mab-0o8d6-822025208484.jpg)
![Immunohistochemistry-Paraffin: Exostosin 1 Antibody [NBP1-91875] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91875-img0009.jpg "Immunohistochemistry-Paraffin: Exostosin 1 Antibody [NBP1-91875] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: Exostosin 1 Antibody [NBP1-91875] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91875-img0007.jpg)
![Immunohistochemistry-Paraffin: Exostosin 1 Antibody [NBP1-91875] - Staining of human placenta shows moderate ctyoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Exostosin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-91875-img0008.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Western Blot: SLC39A8/ZIP8 Antibody [NBP1-76505] - Human spleen tissue lysate with SLC39A8/ZIP8 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SLC39A8-ZIP8-Antibody-Western-Blot-NBP1-76505-img0001.jpg "Western Blot: SLC39A8/ZIP8 Antibody [NBP1-76505] - Human spleen tissue lysate with SLC39A8/ZIP8 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SLC39A8/ZIP8 Antibody - BSA Free [NBP1-76505] - Immunofluorescence of SLC39A8/ZIP8 in human spleen tissue with SLC39A8/ZIP8 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76505_rabbit-polyclonal-slc39a8-zip8-antibody-2711202419531637.jpg)
![Immunohistochemistry: SLC39A8/ZIP8 Antibody - BSA Free [NBP1-76505] - Immunohistochemistry of SLC39A8/ZIP8 in human spleen tissue with SLC39A8/ZIP8 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76505_rabbit-polyclonal-slc39a8-zip8-antibody-271120241931326.jpg)
![Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP3-03712-img0004.jpg "Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded mouse heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0003.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded rat heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0001.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded human colon carcinoma using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0002.jpg)
![Western Blot: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Western blot analysis of extracts of various cell lines, using Calmodulin Rabbit mAb (NBP3-16500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-10W3Y7-Western-Blot-NBP3-16500-img0002.jpg "Western Blot: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Western blot analysis of extracts of various cell lines, using Calmodulin Rabbit mAb (NBP3-16500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (10W3Y7) [NBP3-16500] - Confocal imaging of paraffin-embedded Rat brain using Calmodulin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16500_rabbit-calmodulin-mab-10w3y7-5220251138924.jpg)
![Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (10W3Y7) [Calmodulin] - Confocal imaging of paraffin-embedded Mouse brain using Calmodulin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16500_rabbit-calmodulin-mab-10w3y7-52202517162361.jpg)