| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73452 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89338-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB6000-SP | |||
![Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/RB1-Antibody-172C1094-cleaved-Knockdown-Validated-NB100-56598-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize/cleaved-Retinoblastoma-1-Antibody-172C1094-Immunohistochemistry-NB100-56598-img0004.jpg)
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0007.jpg "Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0004.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS,OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cellsprepared using NETN lysis buffer. Antibody: Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP3-14687] - Whole cell lysate(1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody. For blotting immunoprecipitated E-Cadherin, was used at 1:1000.Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP3-14687-img0001.jpg)
![Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Western-Blot-NBP2-02275-img0003.jpg "Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.")
![Immunocytochemistry/Immunofluorescence: PKMYT1 Antibody (OTI5E1) [NBP2-02275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP2-02275-img0014.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human thyroid tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0012.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Jurkat cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0002.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0004.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0005.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0006.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0007.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human endometrium tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0008.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human Kidney tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0009.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human liver tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0010.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human prostate tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0011.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Hela cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0001.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg)
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg)
![HRAS Antibody (OTI1D9)[NBP2-45745]Simple Western� analysis of endogenous protein MCAM from HELA lysates (0.1 mg/mL) using CD146 (MCAM) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45745_mouse-hras-mab-oti1d9-simple-western-131120251236222.jpg%253fstore=america&image-type=image)
![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0006.jpg "Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of NIH-3T3 cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded human liver cancer using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0002.jpg)
![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of various cell lines, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of HeLa cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded rat spleen using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0001.jpg)
![Immunoprecipitation: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM3 antibody (NBP3-15386). Western blot was performed from the immunoprecipitate using MCM3 antibody (NBP3-15386) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunoprecipitation-NBP3-15386-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (9Z5W4) [MCM3] - Immunofluorescence analysis of NIH-3T3 cells using MCM3 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15386_rabbit-mcm3-mab-9z5w4-52202517235765.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1L4J1-Western-Blot-NBP3-15661-img0010.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![<b>Genetic Strategies Validation. </b>Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-western-blot-305202492857..jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517344271.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251730319.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251731552.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517361213.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517311090.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517352775.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa")
![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NB100-79807-img0011.jpg "Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79807-img0012.jpg)
![Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Western-Blot-MAB6000-img0002.jpg "Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.")
![Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Immunohistochemistry-MAB6000-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - An intracellular stain was performed on HeLa cells with Cyclin B1 Antibody [2061A] MAB6000F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0004.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - K562 human cell line was stained with Rabbit Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB6000, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0001.jpg)