| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26511-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47279-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38371-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79873 | |||
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EML4-Antibody-Western-Blot-NBP1-05973-img0004.jpg "Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EML4 used at a dilution of1:1000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-05973-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EML4 Antibody [NBP1-05973] - Detection of human EML4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EML4 antibody NBP1-05973 (lot NBP1-05973-2) used for IP at 6 ug per reaction. EML4 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05973-1) and rabbit anti-EML4 antibody NBP1-05974. For blotting immunoprecipitated EML4, NBP1-05973 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunoprecipitation-NBP1-05973-img0006.jpg)
![Immunohistochemistry: Aldolase A Antibody (7D8) [NBP3-26511] - Image of Aldolase A Antibody (7D8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-262202415355719.jpg "Immunohistochemistry: Aldolase A Antibody (7D8) [NBP3-26511] - Image of Aldolase A Antibody (7D8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Aldolase A Antibody (7D8) [NBP3-26511] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate. All lanes: Aldolase A Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 46 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-282202411472126.jpg)
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (7D8) [NBP3-26511] - Staining of Hela Cells with Aldolase A Antibody (7D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-28220241323617.jpg)
![Western Blot: SGTA Antibody [NBP2-47279] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/SGTA-Antibody-Western-Blot-NBP2-47279-img0003.jpg "Western Blot: SGTA Antibody [NBP2-47279] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.")
![Immunocytochemistry/Immunofluorescence: SGTA Antibody [NBP2-47279] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SGTA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47279-img0004.jpg)
![Immunohistochemistry: SGTA Antibody [NBP2-47279] - Staining of human thyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SGTA-Antibody-Immunohistochemistry-NBP2-47279-img0002.jpg)
![Whole tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibod [HL3438] (NBP3-48814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48814_rabbit-flightless-i-mab-hl3438-western-blot-141120251835460.jpg "Whole tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibod [HL3438] (NBP3-48814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [HL3438] (NBP3-48814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48814_rabbit-flightless-i-mab-hl3438-western-blot-14112025183379.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibod [HL3438] (NBP3-48814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48814_rabbit-flightless-i-mab-hl3438-western-blot-1411202518352217.jpg)
![Flightless I antibody [HL3438] detects Flightless I protein by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Flightless I stained by Flightless I antibody [HL3438] (NBP3-48814) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48814_rabbit-flightless-i-mab-hl3438-immunocytochemistry-immunofluorescence-1411202518243311.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38371] - Analysis using Anti-PRDX5 antibody NBP2-38371 (A) shows similar pattern to independent antibody NBP2-38370 (B).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38371-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0004.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0005.jpg)
![Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Western Blot Analysis of PC-3 cell lysate using NM23-H2/NME2 Antibody (CPTC-NME2-2).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-CPTC-NME2-2-Western-Blot-NBP2-79738-img0003.jpg "Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Western Blot Analysis of PC-3 cell lysate using NM23-H2/NME2 Antibody (CPTC-NME2-2).")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with NM23-H2/NME2 Antibody (CPTC-NME2-2).](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-CPTC-NME2-2-Immunohistochemistry-Paraffin-NBP2-79738-img0001.jpg)
![Protein Array: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NM23-H2/NME2 Antibody (CPTC-NME2-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-CPTC-NME2-2-Protein-Array-NBP2-79738-img0002.jpg)
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP2-39012-img0002.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0004.jpg)
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP2-39012] - Staining in human prostate and endometrium tissues using anti-ACPP antibody. Corresponding ACPP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP2-39012-img0005.jpg)
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP1-51946-img0006.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP1-51946-img0005.jpg)
![Western Blot: NM23-H2/NME2 Antibody [NBP2-19552] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NME2-Antibody-Western-Blot-NBP2-19552-img0002.jpg "Western Blot: NM23-H2/NME2 Antibody [NBP2-19552] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NM23-H2/NME2 Antibody [NBP2-19552] - Sample (30 ug of whole cell lysate) A: whole zebrafish B: zebrafish heart 15% SDS PAGE NBP2-19552 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-19552_rabbit-polyclonal-nm23-h2-nme2-antibody-9102024201571.jpg)
![Immunohistochemistry: NM23-H2/NME2 Antibody [NBP2-19552] - Immunohistochemical analysis of paraffin-embedded zebrafish muscle, using NM23-H2/NME2 antibody (NBP2-19552) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-19552_rabbit-polyclonal-nm23-h2-nme2-antibody-91020242041279.jpg)
![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32185-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32185-img0003.jpg)
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32185 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32185-img0002.jpg)
![Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ribosomal-protein-S27-Antibody-Western-Blot-NB100-1103-img0003.jpg "Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Ribosomal-protein-S27-Antibody-Immunohistochemistry-Paraffin-NB100-1103-img0001.jpg)