| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13294-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33066 | |||
| IHC-Prediluted | NBP2-44365 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73935 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86805-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80971-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73786 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Septin-9 Antibody [NBP2-13294] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-SEPT9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-13294-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-13294] - Staining of human cell line A-431 shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13294-img0006.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0009.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0010.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0013.jpg)
![Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Western-Blot-NBP2-29439-img0011.jpg "Western Blot: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0010.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0012.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin paraffin breast cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0008.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Immunohistochemistry-Paraffin-NBP2-29439-img0009.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) [NBP2-29439] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ERb455).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ERb455-Flow-Cytometry-NBP2-29439-img0007.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0021.jpg "Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunocytochemistry-Immunofluorescence-NBP2-02182-img0018.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0017.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0002.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0003.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0019.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0005.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0016.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0001.jpg)
![Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunoprecipitation-NBP2-02182-img0020.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 & IMR90 cells. (A) IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, & the pre-immune serum (PI) used as a control for the immunoprecipitation. (B) Western for RPA32-p-Thr21 in both HTC75 & IMR90Ajuba-depleted cells. A non-specific band is marked by a *. Total RPA32 levels are shown at the bottom. (C) Model for the role of Ajuba in repression of ATR. The “?” indicates that the interaction between Ajuba & RPA could be direct or indirect. See text for details. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23755068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02182_mouse-rpa2-mab-oti9a1-310202415291913.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0021.jpg "Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Immunocytochemistry-Immunofluorescence-H00001848-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Immunohistochemistry-Paraffin-H00001848-M01-img0018.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2 Western Blot analysis of DUSP6 expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0012.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2. Western Blot analysis of DUSP6 expression in human placenta.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-Western-Blot-H00001848-M01-img0019.jpg)
![ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-3G2-ELISA-H00001848-M01-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0007.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0010.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0008.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0009.jpg)
![Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.](http://images.novusbio.com/fullsize/EML4-Antibody-Immunohistochemistry-Paraffin-NBP1-86805-img0011.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86805_rabbit-polyclonal-eml4-antibody-304202513101431.jpg)
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80971] - Analysis using Anti-SPARC antibody NBP1-80971 (A) shows similar pattern to independent antibody NBP1-80972 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0010.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human placenta shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0013.jpg)
![Western Blot: SPARC Antibody [NBP1-80971] - Analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80971-img0009.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0011.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0012.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human skin shows moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80971-img0014.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Western-Blot-NBP2-76420-img0001.jpg "Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311310.jpg)
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311923.jpg)
![Immunocytochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE MCF-7 cells by ICC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunocytochemistry-NBP2-76420-img0003.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0004.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) and rabbit anti-CTCF (NB500-177).](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunoprecipitation-NBP2-76420-img0005.jpg)
![Flow Cytometry-CTCF Antibody (BLR041F) [NBP2-76420] -Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] or isotype control (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-flow-cytometry-287202312246..jpg)
![Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Western-Blot-NBP2-03644-img0002.jpg "Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.")
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NBP2-03644-img0014.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0013.jpg)
![Flow Cytometry: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Flow-Cytometry-NBP2-03644-img0001.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0006.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0007.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0009.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RASSF1-Antibody-2B11-Immunohistochemistry-Paraffin-NBP2-03644-img0011.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)