RASSF1 Antibody (OTI2B11) - Azide and BSA Free

Western Blot: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.

Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.

Flow Cytometry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RASSF1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody.

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RASSF1 Antibody (OTI2B11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RASSF1 Antibody (OTI2B11) - Azide and BSA Free (NBP2-03644) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RASSF1 (NP_009113) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RASSF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 38.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RASSF1 Antibody (OTI2B11) - Azide and BSA Free

• 123F2
• NORE2A
• pancreas-specific ras association domain family 1 protein
• Ras association (RalGDS/AF-6) domain family member 1
• ras association domain-containing protein 1
• RASSF1A
• RDA32cardiac-specific ras association domain family 1 protein
• REH3P21
• tumor suppressor protein RDA32
• WUGSC:H_LUCA12.5

Background

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RASSF1