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![Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180] - Western blot analysis of CENPE on different lysates with Rabbit anti-CENPE antibody (NBP3-32180) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 316 kDa Observed band size: 316 kDa Exposure time: 43 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32180_rabbit-cenpe-mab-psh03-54-27820241631644.jpg "Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180] - Western blot analysis of CENPE on different lysates with Rabbit anti-CENPE antibody (NBP3-32180) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 316 kDa Observed band size: 316 kDa Exposure time: 43 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunoprecipitation: CENPE Antibody [NBP1-06506] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENP-E antibody NBP1-06506used for IP at 3 ug/mg lysate. CENP-E was also immunoprecipitated by rabbit anti-CENP-E antibodies NBP1-06504 and NBP1-06505, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/CENPE-Antibody-Immunoprecipitation-NBP1-06506-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CENPE Antibody [NBP1-06506] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENP-E antibody NBP1-06506used for IP at 3 ug/mg lysate. CENP-E was also immunoprecipitated by rabbit anti-CENP-E antibodies NBP1-06504 and NBP1-06505, which recognize upstream epitopes.")
![Western Blot: CENPA Antibody (S04-9A5) [NBP3-19506] - Western blot detection of CENPA in K562, Hela cell lysates using NBP3-19506 (1:1000 diluted). Predicted band size: 16kDa. Observed band size: 16kDa.](http://images.novusbio.com/fullsize/CENPA-Antibody-S04-9A5-Western-Blot-NBP3-19506-img0001.jpg "Western Blot: CENPA Antibody (S04-9A5) [NBP3-19506] - Western blot detection of CENPA in K562, Hela cell lysates using NBP3-19506 (1:1000 diluted). Predicted band size: 16kDa. Observed band size: 16kDa.")
![Western Blot: CENPE Antibody (S06-1G4) [NBP3-19602] - Western blot detection of CENPE in Jurkat lysates using CENPE antibody. Predicted band size: 316kDa. Observed band size: 316kDa.](http://images.novusbio.com/fullsize/CENPE-Antibody-S06-1G4-Western-Blot-NBP3-19602-img0001.jpg "Western Blot: CENPE Antibody (S06-1G4) [NBP3-19602] - Western blot detection of CENPE in Jurkat lysates using CENPE antibody. Predicted band size: 316kDa. Observed band size: 316kDa.")
![ELISA: Human CENPB ELISA Kit (Colorimetric) [NBP2-60615] - Standard curve.](http://images.novusbio.com/fullsize/Human-CENPB-ELISA-Kit-Colorimetric-ELISA-NBP2-60615-img0001.jpg "ELISA: Human CENPB ELISA Kit (Colorimetric) [NBP2-60615] - Standard curve.")
![ELISA: CENPA Antibody (1D4-1A3) [H00001058-M01] - Detection limit for recombinant GST tagged CENPA is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CENPA-Antibody-1D4-1A3-ELISA-H00001058-M01-img0002.jpg "ELISA: CENPA Antibody (1D4-1A3) [H00001058-M01] - Detection limit for recombinant GST tagged CENPA is approximately 0.3ng/ml as a capture antibody.")