| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04961-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05378-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35473-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35113-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2721-0.025mg | |||
![Western Blot: PLK2 Antibody [NBP3-04961] -Analysis of extracts of various cell lines, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-04961_rabbit-polyclonal-plk2-antibody-western-blot-28820238591..jpg "Western Blot: PLK2 Antibody [NBP3-04961] -Analysis of extracts of various cell lines, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Western Blot: PLK2 Antibody [NBP3-04961] -analysis of extracts of various cell lines, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/antibody/nbp3-04961_rabbit-polyclonal-plk2-antibody-western-blot-28820239021..jpg)
![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-7220251245389.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432038.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05378_rabbit-polyclonal-cyclin-e1-p-thr395-antibody-72202512432011.jpg)
![Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.](http://images.novusbio.com/fullsize/c-Myc-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13414-img0002.jpg "Western Blot: c-Myc Overexpression Lysate (Adult Normal) [NBL1-13414] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myc.")
![Western Blot: TLK1 Antibody [NBP3-35473] - Western blot analysis of various lysates using TLK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202415161832.jpg "Western Blot: TLK1 Antibody [NBP3-35473] - Western blot analysis of various lysates using TLK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Human placenta using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202414163051.jpg)
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Rat heart using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-91220241519535.jpg)
![Immunohistochemistry: TLK1 Antibody [NBP3-35473] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using TLK1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35473_rabbit-tlk1-pab-912202415133453.jpg)
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-101220241155590.jpg "Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Rat brain using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-1012202411552895.jpg)
![Western Blot: CCNK Antibody [NBP3-35113] - Western blot analysis of various lysates using CCNK Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-1012202411545572.jpg)
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-101220241233852.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0004.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.")
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0001.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0002.jpg)
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0003.jpg)
![Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Western-Blot-NBP2-73184-img0001.jpg "Western Blot: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.")
![Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) - Azide and BSA Free [NBP2-73184] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by CDKN2B antibody.](http://images.novusbio.com/fullsize/p15INK4b-CDKN2B-Antibody-OTI3B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73184-img0002.jpg)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-278202415242669.jpg "Western Blot: MCM6 Antibody (JE65-68) [NBP3-32579] - Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32579) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32579) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-298202413585583.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM6 Antibody (JE65-68) [NBP3-32579] - Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (NBP3-32579) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32579_rabbit-mcm6-mab-je65-68-29202410234240.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)