| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04946-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03913-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59442-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99648-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99872-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99081-50ul | |||
![Western Blot: NEDD1 Antibody [NB100-55420] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NEDD1-Antibody-Western-Blot-NB100-55420-img0002.jpg "Western Blot: NEDD1 Antibody [NB100-55420] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: KLHL13 Antibody [NBP1-80047] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/KLHL13-Antibody-Western-Blot-NBP1-80047-img0001.jpg "Western Blot: KLHL13 Antibody [NBP1-80047] - Jurkat cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: KLHL13 Antibody [NBP1-80047] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/KLHL13-Antibody-Immunohistochemistry-Paraffin-NBP1-80047-img0002.jpg)
![Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Western-Blot-NBP3-04946-img0001.jpg "Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Kif2a Antibody [NBP3-04946] - Western blot analysis of extracts of various cell lines, using Kif2a antibody (NBP3-04946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Kif2a Antibody-Western Blot-NBP3-04946-img0002.jpg)
![Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/LATS2-Antibody-Western-Blot-NBP3-03913-img0004.jpg "Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates](http://images.novusbio.com/fullsize/Fumarase-Antibody-3F6-Western-Blot-NBP2-59442-img0001.jpg "Western Blot: Fumarase Antibody (3F6) [NBP2-59442] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: 293T cell lysates, Lane 6: K562 cell lysates, Lane 7: Jurkat cell lysates")
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99648-img0002.jpg "Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99648] - Immunofluorescence staining of PRC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99648 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-99648_rabbit-polyclonal-prc1-antibody-23520251628112.png)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0003.jpg "Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.")
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human skin (from donor 1) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. The left panel: tissue incubated with primary antibody](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0001.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.](http://images.novusbio.com/fullsize/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0002.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-88798-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate")
![Immunohistochemistry: Cullin 3 Antibody [NBP2-88798] - Immunohistochemistry with Testis tissue at an antibody concentration of 10ug/ml using anti-CUL3 antibody](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NBP2-88798-img0001.jpg)
![Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-99081-img0002.jpg "Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-99081-img0001.jpg)
